Continuous separation of biotransformation products

生物技术正越来越多地应用于工业，以实现更环保、更可持续的加工。好处可能包括较温和的反应条件，如较低的温度/压力，较少的副产物和较高的特异性。在这种新的方法中，可能会形成后续的复杂混合物，这种方法需要更复杂的下游处理(DSP)，以便分离和纯化目标分子。该项目将开发新的连续流动分离技术，并建立在连续流动中自动测量和pH控制的现有工作基础上。开发了实验室规模的连续固液和液液分离设备，然后在典型的生物转化工作中进行了测试。固/液萃取技术旨在解决诸如从酯和酰胺材料中分离多余的脂肪酸或醇等挑战，以及更广泛地说，从Croda的最终产品中去除不想要的杂质。水溶液通常含有缓冲液、酶、辅因子和副产物，可以回收利用。该项目将着眼于溶液的再利用，以确定材料的积累，并定义净化水平，以实现连续的稳定过程。这将为大规模运行提供数据，以促进该技术的工业应用。Croda正在研究使用酶的生物催化，以及发酵和其他生物转化特殊化学品的途径。提高Croda的分离和提纯能力是人们高度关注的问题。追求更高的可持续性和更高的质量是克罗达寻求更有效的净化方法的动力。这些方法可以更广泛地应用于化学生产，而不仅仅是生物技术。

Biotechnology is being increasingly used in industry to allow greener more sustainable processing. Benefits may include milder reaction conditions such as lower temperature/pressure, fewer side-products and higher specificity. Subsequent complex mixtures may be formed during this new approach which commands more sophisticated downstream processing (DSP) in order to isolate and purify the target molecules. This project will develop new continuous flow separation technologies and build on existing work on automated measurement and pH control in continuous flow. Lab-scale continuous solid-liquid and liquid-liquid separation equipment developed and then tested in representative biotransformation work-ups. Both solid/liquid extraction technology is of interest aiming to address challenges such as separation of excess fatty acids or alcohols from ester and amide materials, and more broadly, removing undesirable impurities from Croda's final products. The aqueous solution, that typically contains buffers, enzymes, cofactors and by products, can be recycled. The project will look at reuse of the solution to determine the accumulation of materials and define the purge level to enable a continuous steady-state process. This will provide data for large-scale operation to facilitate industrial take up of the technology. Croda are investigating biocatalysis using enzymes as well as fermentation and other biotransformation routes to speciality chemicals. Advancing Croda's separation and purification capabilities is of high interest. Striving for higher sustainability and higher quality is motivating Croda to seek more efficient approaches to purification. These methods can be applied more generally in chemical production, not just biotechnology