Mouse Core

鼠标核心的目的将是提供一个集中的面向服务的设施，以确保容量 每个参与的项目都需要培育和维持转基因和基因破坏的小鼠。鼠标核心 还将通过支持BUXCO系统的基础设施来服务于该计划的项目 在小鼠身上进行侵入性抗性和依从性测量，这将被大多数项目使用。这个 鼠标核心还将进行每个项目将使用的呼吸道解剖。正如所有项目所建议的 为了利用这些服务的组合，鼠标核心将是该计划项目的重要组成部分 格兰特。转基因/基因敲除生产单元由我们动物设施内的专用空间支持 并且装备齐全。此外，霍尔茨曼实验室经常使用BUXCO系统并携带 他的实验室里精通这些技术的技术人员将会 成为为本计划中的项目进行这些实验的个人。这个核心是在我们的 已被项目调查员、协查人员和 多年来这一应用领域的核心领导者(Holtzman、Green、Brody、Walter、Shipley)。它的工作人员是 拥有该领域专业知识和生产力的集体广泛记录的人员。核心有 我负责培育了100多个独立的转基因和基因敲除品系，其中 每一个的创始人/嵌合体。许多已经和将由这个核心制造的小鼠将进一步 促进项目负责人之间的协作互动。鼠标核心目前也是 部分由另一项计划项目赠款(Robert M.High，PI)和SCOR赠款(Michael J. Holtzman，PI)。华盛顿大学的其他研究人员也利用了这一核心。因此，这将允许 我们要实现规模经济，降低每生产一只鼠标的成本。

The purpose of the Mouse Core will be to provide a centralized service-oriented facility to insure the capacity of each participating project to produce and maintain transgenic and gene-disrupted mice. The mouse core will also serve the projects of this Program by supporting the infrastructure of the BUXCO system for making invasive resistance and compliance measurements in mice, which will be utilized by most projects. The mouse core will also carry out airway dissections that will be utilized by each project. As all projects propose to utilize a combination of these services, the Mouse Core will be an important part of this Program Project Grant. The transgenic/knockout production unit is supported by dedicated space within our animal facility and is fully equipped. In addition, the Holtzman laboratory routinely utilizes the BUXCO system and carries out isolations of mouse airways, and a technician in his laboratory who is proficient in these techniques will be the individual doing these experiments for projects in this Program. This core was developed in our Pulmonary Division in 1995 and has been extensively utilized by Project Investigators, Co-Investigators, and Core Leaders on this application for many years (Holtzman, Green, Brody, Walter, Shipley). It is staffed by personnel with a collective extensive track record of expertise and productivity in this area. The core has been responsible for generating well over 100 separate transgenic and knockout lines, with multiple founders/chimeras of each. Many of the mice which have been and will be made by this core will further serve to facilitate collaborative interactions amongst Project Leaders. The Mouse Core is currently also supported in part by another Program Project Grant (Robert M. Senior, PI) and SCOR grant (Michael J. Holtzman, PI). The core is also utilized by other investigators at Washington University. Thus, this will allow us to realize an economy of scales and lower production costs per mouse generated.