BIOSENSOR

生物传感器倡议的主要目标是继续为细胞迁移中的关键调节和效应节点开发生物传感器，并使用现有的和拟议的生物传感器来获取可用于 由建模倡议等人开发了一个经过实验验证的细胞迁移理论模型 在模特圈子里。该倡议建立在传感器技术、荧光分析、高 吞吐量显微镜和合作从第一个CMC资助期进入第二阶段， 其中我们计划可视化活细胞中的信号事件，并获取和分析大型微观 数据集。建模将作为与建模倡议的密切合作进行。一位少校 该倡议的重点将是Rho家族监管机构，因为他们控制着关键的移民节点。此外 该倡议将侧重于构建生物传感器和光活化试剂，利用现有的 以及新技术，用于这些信号通路的关键上游和下游成员。 最后，我们对一个特定的、关键的信令进行了详细的实验和数学分析 细胞迁移的途径。

The primary goals for the Biosensor Initiative are to continue to develop biosensors for key regulatory and effector nodes in cell migration and to use existing and proposed biosensors to acquire quantitative data that can be used to develop an experimentally verified theoretical model of cell migration by the Modeling Initiative and others in the modeling community. The Initiative builds on the sensor technologies, fluorescence assays, high throughput microscopy and collaborations from the first CMC funding period to move into Phase II, wherein we plan to visualize signaling events in living cells and to acquire and analyze large microscopic data sets. The modeling will be carried out as a close collaboration with the Modeling Initiative. A major focus of the Initiative will be Rho family regulators since they control key migration nodes. In addition the Initiative will focus on construction of biosensors and photoactivatable reagents, using both existing and new technologies, for key upstream and downstream members of these signaling pathways. In the end we produce a detailed experimental and mathematical analysis of a specific, critical signaling pathway in cell migration.