Photofragmentation MS of Ion Mobility Separated Peptides

描述（由申请人提供）：离子迁移率（IM）是减缓和分离肽离子的完美仪器平台，这一创新使高效的VUV光碎片（IM- otofms /MS）在i期得到证明。VUV光碎片产生了一种近乎完美的技术，用于复杂肽混合物的“按需”结构碎片离子分析，特别是当与已证实的IM区分磷酸化和非磷酸化肽的能力相结合时。此外，最近由lonwerks内部资助的研究[34-37]出人意料地证明了将纳米粒MALDI基质结合到生物组织近表面的有效性。最近，lonwerks的出版物[34-40]和专利[41-44]建立了一种生物组织表面分子探针成像的新方法。因此，从颗粒处理的生物表面简化复杂的激光微探针分子离子光谱是另一个应用，也将受益于离子迁移率分离和随需应变光破碎结构分析的结合。第二阶段将开发独特的IM-oTOFMS/MS硬件，在专门设计的oTOFMS中使用专利的多像素位置敏感探测器。该仪器的增强质量和移动分辨率以及光片段质谱将用于蛋白质组学和组织成像的应用测试。商业化取决于显示只有IMS-oTOFMS/MS可以执行这些应用。

DESCRIPTION (provided by applicant): Ion mobility (IM) is a perfect instrumental platform for slowing and separating peptide ions-this innovation has enabled efficient VUV photofragmentation (IM-oTOFMS/MS) to be demonstrated in phase I. VUV photofragmentation yields a near perfect technology for "on demand" structural fragment ion analysis of complex peptide mixtures especially when combined with the proven capability of IM to distinguish phosphorylated vs. unphosphorylated peptides. In addition, recent internally funded research at lonwerks [34-37] has rather unexpectedly proved the efficacy of incorporating nanoparticulate MALDI matrices into the near surface of biotissues. Recently, lonwerks publications [34-40] and patents [41-44] have established a new approach to molecular microprobe imaging of biotissue surfaces. Thus, the simplification of complex laser microprobe molecular ion spectra from particulate treated biosurfaces is another application which will also benefit from the combination of ion mobility separation followed with on demand photofragmentation structural analysis. Phase II will develop unique IM-oTOFMS/MS hardware using patented multi-pixel position sensitive detectors in a specially designed oTOFMS. Enhanced mass and mobility resolution as well as photofragment MS of this instrument will be tested for applications in proteomics and tissue imaging. Commercialization depends on showing that only IMS-oTOFMS/MS can perform these applications.