Photofragmentation MS of Ion Mobility Separated Peptides

离子淌度分离肽的光裂解质谱

• 批准号: 7128171
• 负责人: Kelley L Waters
• 金额: $ 25.95万
• 依托单位: IONWERKS, INC.
• 依托单位国家: 美国
• 财政年份: 2002
• 资助国家: 美国
• 起止时间: 2002-03-01 至 2008-02-29
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7128171
• 关键词: Photofragmentation; MS; Ion; Mobility; Separated

DESCRIPTION (provided by applicant): Ion mobility (IM) is a perfect instrumental platform for slowing and separating peptide ions-this innovation has enabled efficient VUV photofragmentation (IM-oTOFMS/MS) to be demonstrated in phase I. VUV photofragmentation yields a near perfect technology for "on demand" structural fragment ion analysis of complex peptide mixtures especially when combined with the proven capability of IM to distinguish phosphorylated vs. unphosphorylated peptides. In addition, recent internally funded research at lonwerks [34-37] has rather unexpectedly proved the efficacy of incorporating nanoparticulate MALDI matrices into the near surface of biotissues. Recently, lonwerks publications [34-40] and patents [41-44] have established a new approach to molecular microprobe imaging of biotissue surfaces. Thus, the simplification of complex laser microprobe molecular ion spectra from particulate treated biosurfaces is another application which will also benefit from the combination of ion mobility separation followed with on demand photofragmentation structural analysis. Phase II will develop unique IM-oTOFMS/MS hardware using patented multi-pixel position sensitive detectors in a specially designed oTOFMS. Enhanced mass and mobility resolution as well as photofragment MS of this instrument will be tested for applications in proteomics and tissue imaging. Commercialization depends on showing that only IMS-oTOFMS/MS can perform these applications.

描述(申请人提供)：离子迁移率(IM)是减缓和分离多肽离子的完美仪器平台-这项创新使得高效的真空紫外光碎裂(IM-oTOFMS/MS)能够在第一阶段得到展示。VUV光碎裂产生了一种近乎完美的技术，用于复杂多肽混合物的“按需”结构碎片离子分析，特别是当结合IM被证明的区分磷酸化和非磷酸化多肽的能力时。此外，Lonwerks最近由内部资助的研究[34-37]出人意料地证明了将纳米颗粒MALDI基质结合到生物组织近表面的有效性。最近，Lonwerks的出版物[34-40]和专利[41-44]已经建立了一种新的方法来对生物组织表面进行分子微探针成像。因此，粒子处理生物表面的复杂激光微探针分子离子光谱的简化是另一个应用，它也将受益于离子迁移率分离和按需光碎裂结构分析的组合。第二阶段将开发独特的IM-OTOFMS/MS硬件，在专门设计的OTOFMS中使用专利多像素位置敏感探测器。增强的质量和迁移率分辨率以及该仪器的光碎片MS将在蛋白质组学和组织成像中进行应用测试。商业化取决于表明只有IMS-oTOFMS/MS才能执行这些应用。