The genetics of flowering in raspberry and blackberry - what makes a primocane?

本博士项目的目的是在第一时间了解两种覆盆子类型中樱草和佛洛里康的结实习性和早果性的遗传控制，并研究这些性状在黑莓中的对应关系。为了定位控制这些性状的QTL，学生将利用东马林悬钩子育种协会现有的育种群体。将在整个育种计划中对结果习性进行详细的描述，并将使用SNP标记对最合适的家系进行基因分型。通过这样做，本项目将有助于目前落后于草莓基因组资源的悬钩子属分子工具的开发。本博士项目的目的是首先了解报春藤和花冠结果习性的遗传控制，以及两种树莓类型的结果提前，并研究这些性状在黑莓中的对应关系。为了定位控制这些性状的QTL，学生将利用东马林悬钩子育种协会现有的育种群体。将在整个育种计划中对结果习性进行详细的描述，并将使用SNP标记对最合适的家系进行基因分型。该项目将有助于目前在基因组资源利用方面落后于草莓的悬钩子属植物分子工具的开发。基于QTL作图数据，将开发遗传标记，以促进针对不同结实习性的标记辅助育种。为了更详细地了解开花的控制，将研究不同开花习性的品种中选定的候选基因的表达。此外，基于我们在基因转化方面的强大专业知识，将为树莓开发一种基因编辑协议，并将使用基因编辑品系作为概念验证来测试至少两个候选基因的功能。

The aim of this PhD project is to understand the genetic control of primocane vs. floricane fruiting habit and earliness of fruiting in both raspberry types in the first instance and investigate the correspondence of these traits in blackberry. To map QTLs controlling these traits the student will utilize existing breeding populations available in the East Malling Rubus Breeding Consortium. Detailed characterization of fruiting habits will be carried out throughout the breeding program and the most suitable families will be genotyped for mapping using SNP markers. In doing so, this project will contribute to the development of molecular tools in Rubus which currently lags behind strawberry in availability of genomic resources.The aim of this PhD project is to understand the genetic control of primocane vs. floricane fruiting habit and earliness of fruiting in both raspberry types in the first instance and investigate the correspondence of these traits in blackberry. To map QTLs controlling these traits the student will utilize existing breeding populations available in the East Malling Rubus Breeding Consortium. Detailed characterization of fruiting habits will be carried out throughout the breeding program and the most suitable families will be genotyped for mapping using SNP markers. In doing so, this project will contribute to the development of molecular tools in Rubus which currently lags behind strawberry in availability of genomic resources.Based on QTL mapping data, genetic markers will be developed to facilitate marker assisted breeding targeting different fruiting habits. To understand the control of flowering in more detail, the expression of selected candidate genes will be studied in cultivars with contrasting flowering habits. Moreover, based on our strong expertise in genetic transformation, a gene-editing protocol will be developed for raspberries, and the function of at least two candidate genes will be tested using gene-edited lines as a proof of concept.