Novel Chromatographic Sorbents for Large Biomolecules

项目描述（由申请人提供）：本项目主要研究开发一种新型色谱介质，该介质通过在无纺布上涂覆极薄的聚合物涂层，并具有适合吸附和纯化大分子的离子交换基团而制备。所得到的色谱介质和设备将具有高效率和稳健性的组合，使其使用更有效，更高效，并且比类似应用的现有介质成本更低。具体目标是：(1)优化和开发具有离子交换功能的涂层非织造布家族的化学物质，(2)使用无纺布板材连续涂层设备开发和演示涂层工艺的性能，(3)表征用于重组蛋白质的基材，(4)基于涂层非织造布的特性构建原型设备并表征性能。生物大分子的纯化是生物学研究和生产重组蛋白的重要组成部分，在医学上有许多应用。用于此目的的传统色谱介质具有大孔隙以容纳这些大分子。因此，它们是易碎的和可压缩的，这使得它们在柱层析和生物处理中的使用变得困难。此外，为了尽量减少缓冲流产生的压降，介质的粒度需要大(50-100 ？dia)。颗粒孔内的大颗粒尺寸和小分子扩散系数降低了分离过程的效率。该项目的潜在创新解决了现有技术的固有问题。这项研究将产生新的、低成本的材料和技术，用于纯化和分离重组蛋白和大分子。该项目的潜在商业应用是(a)用于阴离子和阳离子交换色谱的涂层非织造材料，(b)用于亲和色谱的涂层非织造材料，(c)用于放大色谱的低成本一次性设备，以及(d)涉及膜产品的公司的特定应用许可。拟议的研究与公共卫生的相关性是为了加速和改进正在开发的治疗药物的开发，以消除癌症造成的痛苦和死亡。我们需要这个项目的技术来确保新的抗癌疗法的交付和降低成本，比如需要大量的重组蛋白。

DESCRIPTION (provided by applicant): This project is focused on the development of a new type of chromatographic medium prepared by applying very thin polymeric coatings onto non-woven fabrics and having ion exchange groups suitable for adsorption and purification of large biomolecules. The resulting chromatographic media and devices will have a combination of high efficiency and robustness, making their use more effective, more efficient, and at lower cost than existing media for similar applications. The specific aims are to (1) optimize and develop chemistries for a family of coated non-wovens having ion exchange functionalities, (2) develop and demonstrate the performance of the coating process using an apparatus for continuous coating of non-woven sheet stock, (3) characterize substrates for applications to recombinant proteins, (4) build prototype devices based on the properties of the coated non-wovens and characterize performance. Purification of large biomolecules is an important component of biological research and for the production of recombinant proteins utilized for numerous applications in medicine. Conventional chromatographic media for this purpose have large pores to accommodate these large molecules. As a consequence, they are fragile and compressive making their use in column chromatography and bioprocessing difficult. Also, to minimize the compressive pressure drops produced by buffer flow, the particle size of the media need to be large (50-100 ? dia.). The large particle size and small diffusion coefficients of large molecules within the particle pores reduces efficiency of the separation process. Potential innovations of this project address the inherent problems with existing technology. This research will result in new, low cost materials and technology for purification and isolation of recombinant proteins and large biomolecules. Potential commercial applications of this project are (a) coated non-woven materials for anion and cation exchange chromatography, (b) coated non-woven materials for affinity chromatography, (c) low cost, disposable devices for scale-up chromatography, and (d) application specific licenses to companies involved in membrane products. The relevance of the proposed research to public heath is for the accelerated and improved development of therapeutic drugs that are being developed to eliminate suffering and death due to cancer. The technology...from this project is needed to ensure delivery and reduce the cost of new, anti-cancer therapeutics such as recombinant proteins which are needed in large quantities.