A Dual Electrospray/Photoionization Source

描述（由申请人提供）：在第二阶段，我们提出了一个实验计划，将导致商业质量的电喷雾/光电离（ES/PI）源。我们计划：（i）优化和构建用于商业质谱仪的预商业原型ES/PI源，以及（ii）开发和展示突出ES/PI源的独特益处的关键应用，第一阶段项目的目标得到满足，并且在Micromass LCT和Agilent MSD质谱仪上构建和测试原型ES/PI。从第一阶段开始，Syagen进行了额外的测试，并将一个新的源适配到沃茨ZQ。测量ES/PI性能作为流速、加热、PI位置和ES电离（ESI）电压的函数。使用未被ESI有效电离的各种化合物（例如，类固醇、酚类、醛类等）。PI有效地电离了这些化合物。对药物和蛋白质混合物进行了进一步的测试，以检查同时分析两类化合物的潜力。这项工作的意义和重要性是很高的。为生命科学研究开发新的电离源是质谱法的主要推动力，如2002年诺贝尔奖授予ESI和激光解吸/电离（例如，MALDI）。这项工作将极大地扩展ESI的能力，使高通量分析的新的先进方法的发展成为可能。商业MS供应商已经对此技术感兴趣。如果我们在第二阶段的工作中取得成功，我们有信心找到ES/PI来源的商业化合作伙伴。

DESCRIPTION (provided by applicant): In phase II we propose an experimental plan that will lead to a commercial quality electrospray/photoionization (ES/PI) source. We plan to: (i) optimize and build a pre-commercial prototype ES/PI source for use on commercial mass spectrometers, and (ii) develop and demonstrate key applications that highlight the unique benefits of the ES/PI source, The goals of the phase I project were met and a prototype ES/PI was built and tested on a Micromass LCT and an Agilent MSD mass spectrometer. Since phase I, Syagen has performed additional tests, and has adapted a new source to a Waters ZQ. The ES/PI performance was measured as a function of flow rate, heating, PI position, and ES ionization (ESI) voltage. Tests were conducted using a variety of compounds not ionized efficiently by ESI (e.g., steroids, phenols, aldehydes, etc.). PI ionized these compounds efficiently. Further tests were performed on drug and protein mixtures to examine the potential to analyze both classes of compounds simultaneously. The significance and importance of this work is high. Developing new ionization sources for life science research is a major thrust in mass spectrometry as witnessed by the awarding of the 2002 Nobel Prize for ESI and laser desorption/ionization (e.g., MALDI) of biological molecules. This proposed work will greatly expand the capabilities of ESI making possible the development of new advanced methods for high-throughput analysis. There is already interest in this technology by commercial MS vendors. If we are successful with the phase II work, we are confident of finding a commercialization partner for the ES/PI source.