B. subtilis gene expression & replication perturbation

描述(申请人提供)：我的目标是了解DNA复制过程中基因表达的系统性调节。未能及时完成DNA复制可能会导致细胞死亡或基因组不稳定，这通常与癌症有关。在许多生物体中，复制过程中DNA损伤和其他原因的影响通常通过转录反应来减轻。最近，在对受到DNA复制干扰的枯草杆菌细胞的功能基因组分析中，发现了一种新的全局转录反应。这些基因中至少有50个被认为受到DNAA的调控，DNAA是一种必需的、保守的ATPase、转录因子和复制启动子。我计划在枯草杆菌中识别由Dna A直接调控的基因，阐述Dna A是如何作为转录调节因子被激活的，并研究它的目标启动子是如何结构的来对其做出反应的。

DESCRIPTION (provided by applicant): My goal is to understand the systematic modulation of gene expression in response to problems during DNA replication. Failure to complete DNA replication in a timely manner can lead to cell death or genome instability, often associated with cancer. In many organisms, the effects of DNA damage and other causes of distress during replication are often mitigated through transcriptional responses. Recently, a novel global transcriptional response was identified in a functional genomic analysis of Bacillus subtilis cells perturbed in DNA replication. At least 50 of these genes are thought to be regulated by DnaA, an essential and well conserved ATPase, transcription factor, and replication initiator. I plan to identify the genes directly regulated by DnaA in B. subtilis, address how DnaA is activated as a transcriptional regulator, and examine how its target promoters are structured to respond to it.