Dynamics of mRNA Elongation Regulatory Complexes

蛋白质表达水平的改变由许多机制介导，包括响应于不同的外部刺激如营养条件而上调和下调基因转录。此外，这些细胞还可以通过调节转录后过程如翻译来刺激蛋白质水平的产生或抑制。由于这个原因，mRNA表达水平的单独分析不足以代表细胞中蛋白质水平发生的变化。已有资料表明，在S.在酿酒酵母中，包括mRNA延伸复合物的一些成员在内的许多转录调节蛋白的水平响应于基本培养基中的生长而通过转录后机制调节。我们推测，蛋白质含量的这些变化导致了参与细胞凋亡的蛋白质复合物的组成或化学计量的改变。 转录延伸的调节以及其他核过程的调节。使用定量蛋白质组学方法，我们将确定蛋白质表达水平的变化如何改变蛋白质复合物的组成，并分析这些变化如何改变细胞的基因表达谱。

Alterations in protein expression levels are mediated by a number of mechanisms including the up- and down- regulation of the transcription of genes in response to different external stimuli such as nutrient conditions. In addition, these cells can also stimulate the production or repression of protein levels by modulating post-transcriptional processes such as translation. For this reason, the sole analysis of the levels of mRNA expression does not sufficiently represent the changes that occur at the protein level in the cell. Previous data has indicated that in S. cerevisiae, the levels of a number of transcriptional regulatory proteins including some members of mRNA elongation complexes are regulated by post-transcriptional mechanisms in response to growth in minimal media. We hypothesize that these changes in protein content lead to alterations in either the composition or stoichiometry of the protein complexes involved in the regulation of transcriptional elongation as well as the regulation of other nuclear processes. Using a quantitative proteomics approach, we will determine how changes in the level of protein expression alter the makeup of protein complexes and analyze how these changes alter the gene expression profile of the cell.