A study on microplastics interference in mammalian cells metabolism via Infrared Microimaging and Nanospectroscopy

目的是发展一种结合焦平面阵列(FPA)探测器的活细胞同步辐射(SR)傅里叶变换红外(FTIR)成像和固定细胞上的FTIR纳米光谱来研究哺乳动物细胞内微/纳米塑料的分子相互作用的实验方法。微塑料污染已引起科学界和公众的关注。定义为塑料颗粒和纤维&5毫米，这些低密度材料很容易分散在它们持续存在的整个环境中。由于塑料仍然被广泛使用，微塑料和潜在的纳米塑料(&lt；100 nm)粒子在降解和脱落的过程中将继续释放到环境中。虽然有证据表明人们通过食物、饮用水、粉尘和空气接触到微塑料，但关于它们的毒性的基本原理仍不清楚。(Vethaak 2021)考虑到它们发生的多样性，在粒子毒理学原理的指导下，了解急性毒性如何随成分(例如，低/高密度塑料材料)和尺寸(微/纳米)而变化，对于预测风险是相关的。(Riediker，2019年)此外，了解微塑料是否在细胞内起作用，以及这些变量(聚合物类型、尺寸)如何影响这一点，是预测暴露后其影响的潜在长期性的关键。

The aim is to develop an experimental approach combining live cell Syncrotron Radiation (SR) Fourier transform infrared (FTIR) imaging via a Focal plane array (FPA) detector and FTIR nanospectroscopy on fixed cells to study the molecular interactions of micro/nanoplastics within mammalian cells.Microplastic pollution has attracted attention in both the scientific community and general public. Defined as plastic particles and fibres <5 mm, these low-density materials are easily dispersed throughout the environment where they persist. As plastic remains widely used, the release of micro- and potentially nanoplastic (<100 nm) particles to the environment will continue as they degrade and shed. Whilst there is evidence for population exposure to microplastics via food, drinking water, dust and air, fundamentals about their toxicity remain unknown. (Vethaak 2021) Given the variety in which they occur, understanding how acute toxicity varies with composition (e.g., low/high density plastic materials), and size (micro/nano) are pertinent to predicting risk, informed by principals in particle toxicology.(Riediker 2019) Moreover, understanding whether microplastics biopersist inside cells and how these variables (polymer type, size) affect this is key to predicting the potential chronicity of their effects following exposure.