Cyto Vision Workstation For Use In Multi-User Facility

描述（由申请人提供）：该提案要求资金用于购买直立，宽视野，自动化和电动显微镜，以及远程访问工作站，以取代我们目前在凯斯西储大学（CASE）遗传学系共享资源中的不可靠和过时的系统。Applied Imaging，Inc.是CytoVision工作站，由全自动和电动Zeiss Axioplan 2立式显微镜（带x-y-z扫描载物台）、适当的透镜和滤光片、罗珀科学CoolSnap HQ摄像机以及显微镜和远程访问工作站的适当计算机硬件和软件组成。新系统旨在取代一个不适当和过时的Quips基因工作站，该工作站正在失效，并将设施升级为机动化和自动化。它还将满足遗传学系和医学院对系统日益增长的需求，该系统可以一次扫描多达8张载玻片，并在多个载物台位置自动聚焦和自动捕获荧光标记和组织学染色标本的图像。该仪器将存放在当前的共享设施中，用于检查染色体、细胞、组织切片和生物体。成像主任负责系统的日常运作，并将与一个咨询委员会一起监督设施的管理。遗传学部和CASE将提供空间分配、装修、安装、维护和成像主任工资的支持。本项目的实施依赖于多标记荧光和组织学分析。基于技术考虑和仪器之间的比较，CytoVision工作站是满足CASE教师苛刻成像需求的最佳选择。

DESCRIPTION (provided by applicant): This proposal requests funds for the acquisition of an upright, widefield, automated, and motorized microscope, and a remote access workstation, to replace an unreliable and outmoded system we currently have in our shared resource in the Department of Genetics at Case Western Reserve University (CASE). The requested system from Applied Imaging, Inc. is a CytoVision Workstation and consists of a fully automated and motorized Zeiss Axioplan2 upright microscope with an x-y-z scanning stage, appropriate lenses and filters, a Roper Scientific CoolSnap HQ camera, and appropriate computer hardware and software for the microscope and the remote access workstation. The new system is designed to replace an inadequate and outdated Quips Genetic Workstation that is failing, and upgrade the facilities for motorization and automation. It will also meet the increasing demand within the Genetics Department and the School of Medicine for a system that can scan up to 8 slides at a time and autofocus and autocapture images, at multiple stage locations, of both fluorescently-labeled and histologically-stained specimens. The instrument will be housed in the current shared facility for examination of chromosomes, cells, tissue sections, and organisms. The imaging director is responsible for the day-to-day operation of the system and, together with an advisory committee, will oversee management of the facility. The Genetics Department and CASE will provide support for space allocation, renovation, installation, maintenance, and salary for the imaging director. The projects in this proposal depend upon multi-labeled fluorescence and histology analysis. Based on technical considerations and comparisons between instruments, the CytoVision Workstation is the best choice to meet the demanding imaging needs of the faculty at CASE.