AB 3730XL, 96 Cap DNA Analyzer

描述（由申请人提供）：这份向NCRR共享仪器拨款计划提交的提案要求获得资金，用于购买一台自动DNA测序机和数据存储设备，供美国国立卫生研究院资助的海洋生物实验室研究人员共享使用。此次采购的设备包括应用生物系统公司的3730XL DNA分析仪和基于苹果电脑公司的Xserve RAID和Xserve G5架构的3.4兆字节数据存储系统。该设备将满足5个新的NIH项目（去年资助或在2004年5月1日前启动的项目）新的DNA测序要求。这些NIH项目包括寄生原生生物的基因表达序列分析（SAGE）研究，来自多达30个寄生原生生物的大规模cdna进化分析，新伍兹霍尔海洋与人类健康中心（COHH）项目的环境PCR扩增子的序列和序列分析（SARST）分析，以及共生原核生物的小规模基因组研究。这些项目将影响我们对人类病原体基因表达模式、导致疾病的原生生物进化、致病微生物在海洋环境中的扩散以及导致寄生生活方式的适应机制的理解。该设备将使我们目前的测序能力增加近一倍，目前已全部订购。该设备将在海洋生物实验室海湾保罗中心的W.M.凯克生态和进化遗传学设施内运行。该设施是伍兹霍尔科学社区研究人员共享的资源，配备了用于菌落采摘、模板生产、测序反应准备、自动DNA测序和DNA微阵列的机器人系统。自1997年以来，一个用户委员会一直指导该设施的使用，该设施由海湾保罗中心的一名教员管理。基金会已预留资金支付五年的维修费用。

DESCRIPTION (provided by applicant): This proposal to the NCRR Shared Instrumentation Grant Program requests funds to acquire an automated DNA sequencing machine and data storage equipment for shared use by NIH-funded investigators at the Marine Biological Laboratory. The equipment to be purchased includes an Applied Biosystems 3730XL DNA Analyzer and a 3.4 tera-byte data storage system based upon Apple Computer's Xserve RAID and Xserve G5 architecture. The equipment will accommodate new DNA sequencing requirements for five new NIH programs (funded within the last year or projects to be activated by May 1, 2004). These NIH projects include several Serial Analysis of Gene Expression (SAGE) studies in parasitic protists, a large-scale evolutionary analysis of cDNAs from as many as thirty parasitic protists, sequence and Serial Analysis of Ribosomal Sequence Tags (SARST) analyses of environmental PCR amplicons for the new Woods Hole Center for Oceans and Human Health (COHH) program, and small-scale genome studies of symbiotic prokaryotes. These projects will impact our understanding of patterns of gene expression in human pathogens, the evolution of protists that cause disease, the dispersal of pathogenic microrganisms in marine environments, and adaptive mechanisms that lead to parasitic lifestyles. This equipment will nearly double our current sequencing capacity, which is currently fully subscribed. The equipment will operate within the W.M. Keck Ecological and Evolutionary Genetics Facility within the Bay Paul Center at the Marine Biological Laboratory. The facility is a shared-use resource for investigators in the Woods Hole Scientific Community and is equipped with robotic systems for colony picking, template production, preparation of sequencing reactions, automated DNA sequencing and DNA microarraying. Since 1997, a users' committee has guided use of the facility, which is managed by a faculty member in the Bay Paul Center. Foundation funds have been reserved to pay for five years of maintenance.