Metabolic engineering of Escherichia coli for carbon capture and hydrogen storage.

通过合成生物学增强可持续未来的能力：释放工程细菌捕获二氧化碳的潜力，并通过氢储存化学途径释放可持续未来。迅速上升的全球二氧化碳水平造成了迫在眉睫的威胁，需要为可持续的未来和环境复原力采取紧急行动。该项目的重点是设计一种新的底盘菌株大肠杆菌，它将在捕获的二氧化碳来源上生长，包括甲酸和甲醇，作为唯一的碳源。然后，这种新的底盘将被进一步设计，以生产具有工业价值的商品化学品。利用可再生的氢气将废弃的二氧化碳转化为专业生物化学品，将是迈向循环生物经济和实现净零目标的重要一步。该项目将采用三种平行的方法：(1)设计一种依赖氢气的二氧化碳还原酶，其中二氧化碳和氢气将被转化为甲酸。目标：1.优化依赖氢气的二氧化碳还原酶活性。提高合成代谢途径中捕获的碳(甲酸)的利用。(2)合成甲酸的特征，其中甲醇将被转化为甲酸盐。目标：1。使用工程酶证明依赖于甲醇的甲酸盐的生产。将这一途径与合成还原甘氨酸途径整合在一起。(3)设计、构建和测试一种新的厌氧、依赖于H2的还原甘氨酸途径，以促进大肠杆菌在单独的H2和CO2或替代甲醇或甲酸上的生长。目标：1.捕获碳源。利用固定碳进行细菌生长。利用固定碳生产有价值的特种化学品。该项目抓住机遇，介绍了二氧化碳捕获和有价值的化学品生产的途径，通过工程细菌和尖端的氢气储存途径开启了可持续的未来。

Empowering a sustainable future through Synthetic Biology: Unleash engineered bacteria's potential for carbon dioxide capture and unlock a sustainable future through hydrogen storage chemical pathways. Rapidly increasing global carbon dioxide levels cause an imminent threat, demanding urgent action for a sustainable future and environmental resilience. This project is focused on engineering a new chassis strain of Escherichia coli that will grow on sources of captured carbon dioxide, including formic acid and methanol, as sole carbon sources. This new chassis will then be further engineered to produce commodity chemicals with an industrial value. Converting waste CO2 into specialist biochemicals using renewable H2 will be a major step towards a circular bioeconomy and achieving Net Zero targets. The project will take three parallel approaches: (1) Engineering of a hydrogen-dependent CO2 reductase where the CO2 and H2 will be converted to formate.Objectives:1. Optimise hydrogen-dependent CO2 reductase activity.2. Enhance captured carbon (formic acid) utilisation in synthetic metabolic pathways.(2) Characterisation of synthetic methylotrophy where the methanol will be converted to formate.Objectives:1. Demonstrate methanol-dependent formate production using engineered enzymes.2. Integrate this pathway with the synthetic reductive glycine pathway.(3) Design, build and test of a novel anaerobic, H2-dependent reductive glycine pathway to facilitate E. coli growth on H2 and CO2 alone, or alternatively methanol or formic acid. Objectives:1. Capturing carbon.2. Utilisation of fixed carbon for bacterial growth.3. Utilisation of fixed carbon for valuable speciality chemicals.Seizing the opportunity, this project introduces pathways for CO2 capture and valuable chemical production, unlocking a sustainable future through engineered bacteria and cutting-edge hydrogen storage pathways.