CORE--AGING TRANSGENIC RODENT/PATHOLOGY

老龄转基因啮齿动物/病理核心(ATRPC)是由埃文·凯勒领导的。ATRPC的主要目的是为内森休克中心(NSC)和密歇根大学(UM)的科学家提供老龄小鼠的基因型别，这些基因型别提供了研究衰老的基本生物学或与年龄相关疾病状态的病理生理学的机会。国家安全委员会在密歇根大学运营的ATRPC这样的设施在该国其他任何地方都不存在。ATRPC的S MTRC主要致力于利用现有的广泛的突变和转基因，但尚未在老龄化的背景下进行研究，用于老年学研究。ATRPC作为服务核心发挥作用，具体目标如下： 1)支持各种突变/转基因啮齿动物老化的每日费用，(2)促进转基因小鼠与年龄相关损害的大体尸检和组织病理学，以及(3)创建可供NSC和UM调查人员使用的转基因小鼠数据库。在过去五年的支持中，ATRPC AS支持了13个项目，有12个教师赞助商。大多数项目的资助时间为2年，这是在研究所需的年龄获得小鼠所需的通常时间。在当前授权期内获得资助的项目包括：评估衰老对甲基转移酶基因敲除小鼠甲基化的影响、确定衰老对人源化雄激素受体的影响、评估WNT与骨骼中的AGE以及评估雌激素受体随年龄的突变。这些项目中有几个已经出版并申请赠款。ATRPC已经有了一个重要的 对密歇根大学校园的NSC科学家以及他们在密歇根大学和其他地方的同事们使用转基因小鼠的影响。

The Aging Transgenic Rodent/Pathology Core (ATRPC) is directed by Evan Keller. The major purpose of the ATRPC is to provide Nathan Shock Center (NSC) and University of Michigan (UM) scientists with aged mice of the genotypes that provide otherwise unavailable opportunities to investigate the basic biology of aging or the pathophysiology of age-related disease states. A facility such as the ATRPC operated by the NSC at the UM does not exist anywhere else in the country. The ATRPC' s MTRC is primarily dedicated to exploiting the wide range of mutant and transgenics currently available, but not yet studied in the context of aging, for gerontological research . The ATRPC functions as a service core with the following specific aims: 1) the support of the per diem costs of aging a wide variety of mutant/transgenic rodents, (2) facilitating gross necropsy and histopathology of age-related lesions for genetically ~ modified mice, and (3) creating a database of the genetically -modified mice that are available for NSC and UM investigators. Over the previous five years of support, the ATRPC as supported thirteen projects with 12 faculty sponsors. Most projects were funded for 2 years, which was the usual time required to obtain mice at the ages required for the study. The projects funded during the current granting period included: evaluating aging effects on methylation in a methyltransferase knockout mice, determining aging effects on a humanized androgen receptor in mice, evaluation of wnt with age in bone and evaluation of estrogen receptor mutations with age. Several of these projects have led to publications and grant applications. The ATRPC has had a significant impact on the use of genetically-modified mice for NSC scientists on the UM campus and among their colleagues at UM and elsewhere.