Neural Development and Genetics of Zebrafish

描述（由申请人提供）： 该提案要求为高级研究生，博士后研究员和独立调查人员提供为期两周的强化讲座/实验室课程的部分支持，重点是斑马鱼的发育和遗传学，特别强调神经系统。课程的第一周涵盖斑马鱼的早期发育和遗传学以及相关方法，而第二周则侧重于神经系统。上午和下午通常用于背景讲座和实验练习，晚上用于研究讲座。每一天都是在一名高级教师的监督下，由一名或两名初级教师协助。 在第一周，学生学习RNA和反义吗啉代注射，转基因，镶嵌分析，和整体安装RNA和抗体原位杂交，标准的任何斑马鱼实验室的基本技术。第一周的第二个重点是斑马鱼遗传学，包括诱变、遗传筛选方法和定位克隆。课程的第二周侧重于斑马鱼神经系统的发育和功能，尽管许多技术适用于研究其他器官系统的发育。例如，学生被教导各种技术注射，标记和移植单细胞，以评估基因功能，细胞命运，或细胞行为在以后的发展阶段。课程的最后几天教授在基因和神经回路水平上研究复杂行为的技术。穿插在实验室会议中的是“圆桌会议”，教授斑马鱼研究的实践方面，例如如何建立和维护斑马鱼设施，如何繁殖斑马鱼，以及如何将它们从卵培养到成年。

DESCRIPTION (provided by applicant): This proposal requests partial support for an intensive two-week lecture/laboratory course for advanced graduate students, postdoctoral fellows and independent investigators that focuses on the development and genetics of zebrafish with special emphasis on the nervous system. The first week of the course covers the early development and genetics of zebrafish and the relevant methodologies, whereas the second week focuses on the nervous system. Mornings and afternoons are generally devoted to background lectures and laboratory exercises and evenings to research lectures. Each day is under the supervision of a senior faculty member who is assisted by one or two junior faculty members. In the first week students learn basic techniques of RNA and antisense morpholino injections, transgenesis, mosaic analysis, and whole-mount RNA and antibody in situ hybridizations, standard to any zebrafish laboratory. A second major focus of the first week is zebrafish genetics, with sessions on mutagenesis, genetic screening methodologies and positional cloning. The second week of the course focuses on zebrafish nervous system development and function, although many of the techniques are applicable to studying the development of other organ systems. For example, students are taught a variety of techniques for injecting, labeling and transplanting single cells, in order to assess gene function, cell fate, or cell behaviors during later developmental stages. The final days of the course teach techniques for studying complex behaviors, both at the levels of genes and of neural circuits. Interspersed with the laboratory sessions, are "round table" sessions that teach the practical side of zebrafish research, such as how to build and maintain a zebrafish facility, how to breed zebrafish, and how to raise them from egg to adult.