Core--Chimerism Analysis and Canine Histocompatibility

这个核心是为了满足所有四个研究项目的需要而设计的。核心人员由核心主任、一名全职技术人员和一名兼职研究技术人员组成。I核有两个组成部分。其中一个是在非清髓调节和造血细胞移植后对狗（项目1和2）和人类患者（项目3和4）进行嵌合分析。这些分析将使用基于聚合酶链反应（PCR）的微卫星标记多态性分析进行。这些研究将用于监测所有四种供体移植的程度

This core is designed to meet the needs of all four research projects on this Program Project Grant. The core personnel consist of the Core Director, one full-time Technician and one half-time Research Technician. The I core has two components. One will be to provide chimerism analyses in dogs (Projects 1 and 2) and human patients (Projects 3 and 4) after nonmyeloablative conditioning and hematopoietic cell transplantation. These analyses will be performed using polymerase chain reaction (PCR)-based microsatellite marker polymorphism assays. These studies will serve to monitor the degree of donor engraftment in all four research projects. The second goal will be to provide canine histocompatibility typing for Projects 1 and 2 with the aim of selecting dog leukocyte antigen (DLA) identical littermate donor-recipient pairs for transplantation.