CORE--MORPHOLOGY, IMMUNOLOGY AND MOLECULAR BIOLOGY

形态学，免疫学和分子生物学核心将提供该计划中的各种项目 具有必要的专业知识，设施和支持，以执行路由光学显微镜，免疫荧光， 显微镜及其他常规和专门的形态学、病理学和免疫组化 需支助 核心的分子生物学部分将包括DNA，蛋白质和蛋白质的生长和纯化。 重组病毒; cDNA的定点诱变，转染载体和质粒的产生， 哺乳动物细胞培养物中通道蛋白的表达和分析。核心也将是主要的 用于生产和维护转基因小鼠群体的设施。这些责任将 包括育种、基因分型和通过免疫化学方法进行表型分析。

The Morphology, Immunology and Molecular Biology Core will provide the various projects in the program with the necessary expertise, facilities, and support to perform routing light microscopy, immunofluorescent, microscopy, and other routine and specialized morphological, pathological and immunohistochemical support required The Molecular Biology component of the Core will include the growth and purification of DNA, proteins and recombinant viruses; site directed mutagenesis of cDNA, production of transfection vectors and plasmids, expression and analysis of channel proteins in mammalian cell cultures. The core will also be the primary facility for the production and maintenance of the transgenic mouse colonies. These responsibilities will include breeding, genotyping and phenotypic analysis by immunochemical means.