MULTIPHOTON CONFOCAL MICROSCOPE UPGRADES: CARDIOVASCULAR

这个子项目是利用由NIH/NCRR资助的共享仪器赠款提供的资源的许多研究子项目之一。子项目和调查员(PI)可能从另一个NIH来源获得了主要资金，因此可能会出现在其他CRISE条目中。列出的机构是用于拨款的，而不一定是用于调查人员的机构。描述(申请人提供)：这是一份升级我们的单光子和多光子激光扫描显微镜系统的建议书，该系统基于我们的蔡司510NLO系统。此次升级包括1)Chameleon，一款易于使用的飞秒脉冲钛宝石激光器，用于取代我们老化且难以使用的Ar泵浦Mira激光器；2)用于510系统的蔡司生理学软件，它可以实现更复杂的图像处理和分析；3)工作站软件，用于在独立计算机上复制510分析功能，以便我们频繁使用的510系统能够用于收集数据。我们的蔡司510系统位于一个使用量很大的多用户设施中，可为各种单光子和多光子激发项目提供图像采集和分析。这些升级是为了1)提高多光子激发的可靠性和易用性，以便普通用户能够独立工作；2)大大提高改变多光子激发的波长的速度，以允许新的实验方法；3)在510系统上提供更多的软件功能；以及4)使分析功能可用，以便510系统可以专门用于数据采集。我们的设施包括另外两台激光扫描共聚焦显微镜，一台蔡司410和一台BioRad 600，以及一台旋转圆盘共聚焦显微镜，来自Atto Instruments。我们的显微镜系统被来自多个实验室的研究人员用于各种项目。

This subproject is one of many research subprojects utilizing the resources provided by a Shared Instrumentation Grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the grant, which is not necessarily the institution for the investigator. DESCRIPTION (provided by applicant): This is a proposal to upgrade our one- and multi-photon laser scanning microscope system that is based on our Zeiss 510 NLO system. The upgrade consists of 1) the Chameleon, an easily use femtosecond pulse Ti:Sapphire laser to replace our aging and difficult to use Ar pumped Mira laser; 2) Zeiss Physiology software for the 510 system that allows more sophisticated image processing and analysis; 3) workstation software to duplicate the 510 analysis functionality on standalone computers so that our heavily used 510 system can be used for collecting data. Our Zeiss 510 system is in a heavily used multi-user facility that provides image acquisition and analysis for a wide variety of one- and multi-photon excitation projects. These upgrades are sought 1) to improve the reliability and ease of use of multi-photon excitation so that an average user can work independently; 2) greatly increase the speed of changing wavelengths of multi-photon excitation to allow novel experimental approaches; 3) provide increased software functionality on the 510 system; and 4) to make analysis functions available "off-line" so that the 510 system can be dedicated to data acquisition. Our facility includes two other laser scanning confocal microscopes, a Zeiss 410 and a BioRad 600, and a spinning disk confocal microscope, from Atto Instruments. Our microscope systems are used for a variety of projects by investigators from several laboratories.