MULTIPHOTON CONFOCAL MICROSCOPE UPGRADES: LIVER, TRANSPLANTATION

该子项目是利用由NIH/NCRR资助的共享仪器补助金提供的资源的众多研究子项目之一。子项目和研究者（PI）可能已经从另一个NIH来源获得了主要资金，因此可以在其他CRISP条目中表示。列出的机构是资助机构，不一定是研究者的机构。描述（由申请人提供）：这是一份基于蔡司510 NLO系统的单光子和多光子激光扫描显微镜系统的升级方案。升级包括1)变色龙，一种易于使用的飞秒脉冲Ti：蓝宝石激光器，以取代我们老化和难以使用Ar泵浦Mira激光器；2)蔡司生理软件为510系统，允许更精密的图像处理和分析；3)工作站软件，在独立计算机上复制510分析功能，以便我们大量使用的510系统可以用于收集数据。我们的蔡司510系统是一个广泛使用的多用户设施，为各种单光子和多光子激发项目提供图像采集和分析。这些升级寻求1)提高多光子激发的可靠性和易用性，使普通用户可以独立工作；2)大大提高了多光子激发波长变化的速度，允许新的实验方法；3)在510系统上提供增加的软件功能；4)使分析功能“离线”可用，使510系统可以专门用于数据采集。我们的设备包括另外两台激光扫描共聚焦显微镜，一台蔡司410和一台BioRad 600，以及一台Atto Instruments的旋转盘共聚焦显微镜。我们的显微镜系统被几个实验室的研究人员用于各种项目。

This subproject is one of many research subprojects utilizing the resources provided by a Shared Instrumentation Grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the grant, which is not necessarily the institution for the investigator. DESCRIPTION (provided by applicant): This is a proposal to upgrade our one- and multi-photon laser scanning microscope system that is based on our Zeiss 510 NLO system. The upgrade consists of 1) the Chameleon, an easily use femtosecond pulse Ti:Sapphire laser to replace our aging and difficult to use Ar pumped Mira laser; 2) Zeiss Physiology software for the 510 system that allows more sophisticated image processing and analysis; 3) workstation software to duplicate the 510 analysis functionality on standalone computers so that our heavily used 510 system can be used for collecting data. Our Zeiss 510 system is in a heavily used multi-user facility that provides image acquisition and analysis for a wide variety of one- and multi-photon excitation projects. These upgrades are sought 1) to improve the reliability and ease of use of multi-photon excitation so that an average user can work independently; 2) greatly increase the speed of changing wavelengths of multi-photon excitation to allow novel experimental approaches; 3) provide increased software functionality on the 510 system; and 4) to make analysis functions available "off-line" so that the 510 system can be dedicated to data acquisition. Our facility includes two other laser scanning confocal microscopes, a Zeiss 410 and a BioRad 600, and a spinning disk confocal microscope, from Atto Instruments. Our microscope systems are used for a variety of projects by investigators from several laboratories.