PURCHASE OF AN 800 MHZ NMR SPECTROMETER

该子项目是利用由NIH/NCRR资助的共享仪器赠款提供的资源的许多研究子项目之一。子项目和研究者（PI）可能从另一个NIH来源获得主要资金，因此可以在其他CRISP条目中表示。列出的机构是用于资助的，不一定是研究者的机构。描述（由申请人提供）：申请资金用于共享800 MHz NMR光谱仪，用于溶液中的大分子。光谱仪的增加将极大地方便正在使用或计划在其工作中使用现代NMR技术的几个研究小组的研究。这些研究小组正在开展一系列广泛的项目，包括研究细菌趋化过程中参与细菌信号传导的蛋白质的结构、功能和动力学以及相关的信号传导途径;蛋白质折叠动力学和未折叠状态的性质; RNA-RNA识别单元的自组装结构;细菌DMA胞嘧啶甲基转移酶中蛋白质动力学和催化作用的耦合;参与编码存在于肾盂肾炎相关皮利中的蛋白质的基因表达的表观遗传控制的蛋白质的结构研究; tau蛋白的结构和组装;以及通过细菌细胞表面展示选择的蛋白质和高亲和力肽配体的复合物的结构。

This subproject is one of many research subprojects utilizing the resources provided by a Shared Instrumentation Grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the grant, which is not necessarily the institution for the investigator. DESCRIPTION (provided by applicant): Funding is requested for a shared 800 MHz NMR spectrometer for work with macromolecules in solution. The addition of spectrometer will greatly facilitate the research of the several research groups that are using or plan to use modern NMR techniques in their work. These groups are working on a wide range of projects that include investigations of the structure, function and dynamics of the proteins involved in bacterial signaling during bacterial chemotaxis and related signaling pathways; protein folding dynamics and the nature of the unfolded state; the structure of self assembly of RNA-RNA recognition units; the coupling of protein dynamics and catalysis in the bacterial DMA cytosine methyltransferase; structural studies of the proteins involved in the epigenetic control of the expression of genes that encode proteins present in pyelonephritis-associated pili; the structure and assembly of tau protein; and the structures of complexes of proteins and high affinity peptide ligands selected by bacterial cell surface display.