Purchase of an 800 MHz NMR spectrometer

描述(由申请者提供)：为一个共享的800 MHz核磁共振波谱仪申请资金，用于处理溶液中的大分子。光谱仪的增加将极大地方便正在或计划在工作中使用现代核磁共振技术的几个研究小组的研究。这些小组开展的广泛项目包括：研究在细菌趋化过程中参与细菌信号传递的蛋白质的结构、功能和动态，以及相关的信号途径；蛋白质折叠动力学和未折叠状态的性质；RNA-RNA识别单元的自组装结构；细菌DMA胞嘧啶甲基转移酶中蛋白质动力学和催化作用的耦合；对肾盂肾炎相关菌毛中编码蛋白质的基因表达的表观遗传控制的蛋白质的结构研究；tau蛋白的结构和组装；以及通过细菌细胞表面展示选择的蛋白质和高亲和力多肽配体的复合体的结构。

DESCRIPTION (provided by applicant): Funding is requested for a shared 800 MHz NMR spectrometer for work with macromolecules in solution. The addition of spectrometer will greatly facilitate the research of the several research groups that are using or plan to use modern NMR techniques in their work. These groups are working on a wide range of projects that include investigations of the structure, function and dynamics of the proteins involved in bacterial signaling during bacterial chemotaxis and related signaling pathways; protein folding dynamics and the nature of the unfolded state; the structure of self assembly of RNA-RNA recognition units; the coupling of protein dynamics and catalysis in the bacterial DMA cytosine methyltransferase; structural studies of the proteins involved in the epigenetic control of the expression of genes that encode proteins present in pyelonephritis-associated pili; the structure and assembly of tau protein; and the structures of complexes of proteins and high affinity peptide ligands selected by bacterial cell surface display.