RESEARCH IRRADIATOR

该子项目是利用由NIH/NCRR资助的共享仪器赠款提供的资源的许多研究子项目之一。子项目和研究者（PI）可能从另一个NIH来源获得主要资金，因此可以在其他CRISP条目中表示。列出的机构是用于资助的，不一定是研究者的机构。描述（由申请人提供）：要求为Nordion Gammacell 40研究辐照器提供资金。该辐照器包含两个1，800 Ci Cs-137源，产生的中心剂量率为1.3戈伊/分钟，约15%。在260 mm直径和100 mm高度上，典型的剂量均匀性为<$6.5%。伽马单元40的腔室尺寸为312 mm × 100 mm。还将购买Nordion衰减器组，这将使我们能够将辐照剂量率降低80%、60%或33%，从而可以根据不同应用改变剂量率。辐照器将用于辐照细胞，如用作抗原呈递细胞来源的脾细胞，并消融小鼠的骨髓细胞，使其接受供体骨髓移植（嵌合体）。目前，蒙大拿州立大学没有可用的辐照器，我们所知道的最近的辐照器是在蒙大拿州汉密尔顿的NIAID落基山实验室，距离我们的校园约260英里。因此，我们需要这种辐照器来利用最先进的免疫学技术，并充分利用转基因小鼠在生物医学研究中的潜在用途。

This subproject is one of many research subprojects utilizing the resources provided by a Shared Instrumentation Grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the grant, which is not necessarily the institution for the investigator. DESCRIPTION (provided by applicant): Funds are requested for a Nordion Gammacell 40 research irradiator. The irradiator contains dual 1,800 Ci Cs-137 sources and produces a central dose rate of 1.3 Gy/min. ¿ 15%. Typical dose uniformity is ¿ 6.5% over a 260 mm diameter and a 100mm height. The Gamma cell 40 has a chamber size of 312 mm by 100 mm. The Nordion attenuator set will also be purchased which will allow us to reduce the dose rate of irradiation by 80, 60, or 33% so that dose rates can be varied for different applications. The irradiator will be used to both irradiate cells, such as spleen cells to be used as a source of antigen-presenting cells, and to ablate bone marrow cells of mice to make them receptive to donor bone marrow engraftment (chimeras). Currently there is no irradiator available at Montana State University and the closest irradiator we know of is at the NIAID Rocky Mountain Laboratories in Hamilton, Montana, which is about 260 miles from our campus. Thus, this irradiator is needed for us to take advantage of state-of-the art immunologic techniques as well as to take full advantage of the potential use of transgenic mice in biomedical investigations.