Research Irradiator

描述(由申请者提供)：申请资金购买Nordion Gammacell 40研究型辐照器。该辐照器包含两个1800Ci Cs-137源，产生的中心剂量率为1.3Gy/min。？15%。典型的剂量均匀度为直径260 mm、高度100 mm以上的6.5%。伽马室40具有312 mm×100 mm的腔尺寸。还将购买Nordion衰减器装置，这将使我们能够将辐射剂量率降低80%、60%或33%，以便可以根据不同的应用而改变剂量率。该辐射器将用于照射细胞，如用作抗原呈递细胞来源的脾细胞，以及去除小鼠的骨髓细胞，使其能够接受捐赠者的骨髓移植(嵌合体)。目前，蒙大拿州立大学没有辐射器，我们所知最近的辐射器是位于蒙大拿州汉密尔顿的NIAID落基山实验室，它距离我们的校园约260英里。因此，我们需要这种辐照器来利用最先进的免疫学技术，以及充分利用转基因小鼠在生物医学研究中的潜在用途。

DESCRIPTION (provided by applicant): Funds are requested for a Nordion Gammacell 40 research irradiator. The irradiator contains dual 1,800 Ci Cs-137 sources and produces a central dose rate of 1.3 Gy/min. ¿ 15%. Typical dose uniformity is ¿ 6.5% over a 260 mm diameter and a 100mm height. The Gamma cell 40 has a chamber size of 312 mm by 100 mm. The Nordion attenuator set will also be purchased which will allow us to reduce the dose rate of irradiation by 80, 60, or 33% so that dose rates can be varied for different applications. The irradiator will be used to both irradiate cells, such as spleen cells to be used as a source of antigen-presenting cells, and to ablate bone marrow cells of mice to make them receptive to donor bone marrow engraftment (chimeras). Currently there is no irradiator available at Montana State University and the closest irradiator we know of is at the NIAID Rocky Mountain Laboratories in Hamilton, Montana, which is about 260 miles from our campus. Thus, this irradiator is needed for us to take advantage of state-of-the art immunologic techniques as well as to take full advantage of the potential use of transgenic mice in biomedical investigations.