ELECTRON MICROSCOPE: DIABETES, NUTRITION, CVD, AGING, MALARIA

该子项目是利用由NIH/NCRR资助的共享仪器赠款提供的资源的许多研究子项目之一。子项目和研究者（PI）可能从另一个NIH来源获得主要资金，因此可以在其他CRISP条目中表示。列出的机构是用于资助的，不一定是研究者的机构。描述（由申请人提供）：密苏里州电子显微镜核心设施的大学提供了唯一的电子显微镜服务和仪器的密苏里州校园的大学研究。正是通过这个核心设施，主要用户群进行研究，需要透射电子显微镜。目前在核心设施中的透射电子显微镜（TEM）是大约20年前购置的。制造商已通知我们，该仪器不再提供新的更换部件。仪器继续使用旧部件进行维修，但一旦任何关键部件的供应耗尽，仪器可能很快变得无法使用。此外，现有显微镜不具有用户组所需的新仪器上可用的某些功能。该应用程序的目标是用现代TEM取代旧仪器，该TEM可以配备满足主要用户组和其他NIH资助的研究人员在未来15至20年内的研究需求的功能。在核心中使用的绝大多数TEM是用于NIH资助的研究。核心TEM服务目前用于支持糖尿病，衰老，心血管疾病，营养，神经退行性疾病和各种其他健康问题的研究。目前安置在核心的TEM将不再能够得到服务的时间即将到来。在没有可靠的替换零件供应的情况下，完全失去TEM能力的风险很高。所要求的TEM将设在现有的核心设施内，并由经验丰富的核心工作人员进行维护。核心工作人员将在电子显微镜服务学院协调员的指导下，与用户咨询委员会协商，监督仪器的使用。仪器使用培训将由核心工作人员和相关教师提供。工作人员的薪金将继续由使用费和大学资金共同提供。将与制造商签订服务合同，为核心人员无法执行的仪器提供服务。服务合同费用将由用户费和必要时的机构补贴支付。该仪器将按照适用于主要用户群体的基本相同条款向主要用户群体以外的用户提供。扩大用户基础将有助于尽可能降低每个用户维护仪器的费用。

This subproject is one of many research subprojects utilizing the resources provided by a Shared Instrumentation Grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the grant, which is not necessarily the institution for the investigator. DESCRIPTION (provided by applicant): The University of Missouri Electron Microscopy Core facility provides the only electron microscopy services and instrumentation for research on the University of Missouri campus. It is through this Core Facility that the major user group performs research that requires transmission electron microscopy. The transmission electron microscope (TEM) currently in the Core Facility was acquired approximately 20 years ago. We have been informed by the manufacturer that new replacement parts are no longer available for this instrument. The instrument continues to be serviced with used parts, but once the supply of any critical part is exhausted, the instrument could soon become nonfunctional. In addition, the existing microscope does not have some features available on newer instruments that are needed by the user group. The objective of this application is to replace the old instrument with a modern TEM that can be equipped with features that will meet the research needs of the major user group and other NIH-funded investigators over the coming 15 to 20 years. The great majority of TEM use in the Core is for NIH-funded research. Core TEM services are currently used to support research on diabetes, aging, cardiovascular disease, nutrition, neurodegenerative disorders, and a variety of other health problems. The time is near when the TEM presently housed in the Core will no longer be able to be serviced. In the absence of a reliable supply of replacement parts, the risk of losing TEM capability altogether is high. The requested TEM will be housed in the existing Core Facility and will be maintained by experienced Core staff. Core staff will supervise use of the instrument under the direction of a Faculty Coordinator for Electron Microscopy Services in consultation with a user Advisory Committee. Training in instrument use will be provided by Core staff and associated faculty. Staff salaries will continue to be provided from a combination of user fees and University funds. A service contract will be maintained with the manufacturer to provide service for the instrument that cannot be performed by Core staff. Service contract costs will be covered by user fees and an institutional subsidy if necessary. The instrument will be available to users outside of the major user group under essentially the same terms that apply to major user group. Broadening the user base will help make the cost per user of maintaining the instrument as low as possible.