MAGNETIC SECTOR FIELD HIGH RESOLUTION MASS SPECTROMETER

该子项目是利用由NIH/NCRR资助的共享仪器赠款提供的资源的许多研究子项目之一。子项目和研究者（PI）可能从另一个NIH来源获得主要资金，因此可以在其他CRISP条目中表示。列出的机构是用于资助的，不一定是研究者的机构。描述（由申请人提供）：（修订摘要）申请资金用于Finnigan Element 2扇形磁场高分辨率电感耦合等离子体质谱仪（HR-ICP/MS）。Element 2是高分辨率多元素/同位素鉴定和定量的最灵敏的分析仪器。所要求的仪器将作为一个国家的最先进的生物分析的核心资源，一个多样化的网络，高生产力，创新的生物医学研究人员在哈佛医学院（HMS）/哈佛公共卫生学院（HSPH）谁需要痕量/超痕量分析能力，为广泛的生物医学，金属组学和金属蛋白生物化学应用。扇形磁场技术超越了基于四极杆的系统，提供了明确的质量分辨率、多元素/同位素灵敏度、多原子无干扰检测和高通量性能，是地球化学、材料科学和半导体行业中高分辨率多元素/同位素应用的公认标准。在生物医学研究中利用这项技术才刚刚开始，并允许金属组学测定，同位素比稀释/示踪实验，临床毒理学/生物标志物流行病学分析，以前没有实现。世界上很少有HR-ICP/MS仪器专门用于生物医学/金属组学研究，在美国也没有。所要求的工具将被用作位于波士顿布里格姆妇女医院的机构间合作核心资源。该仪器将由一个在生物医学痕量金属分析（包括ICP/MS）方面拥有20多年经验/专业知识的分析团队操作。该设施将致力于HMS/HSPH系统内的生物医学金属组学研究，并将提供给其他区域机构的研究人员。仪器选址充分利用了靠近主要核心设施的优势，包括痕量金属分析仪（HSPH）、哈佛医学研究所（HMS）、哈佛遗传学和基因组学中心、哈佛神经疾病中心、衰老、遗传学和神经变性中心以及Taplin生物质谱设施（HMS）。主要用户组包括在细胞/分子生物学，金属蛋白生物化学，生理学，环境毒理学和流行病学，生物金属代谢/稳态，眼科学，泌尿学，胃肠病学和神经精神病学研究前沿的跨学科团队。该仪器的获得将推动阿尔茨海默病、炎症和肿瘤疾病、白内障、神经发育、高血压和环境毒理学的前沿研究。首席研究员和咨询委员会将促进整个地理区域的研究人员公平获得仪器和以具有成本效益的方式使用仪器。我们预计，这一核心设施资源将促进生物医学金属组学的发现，并加快在这一新领域前沿建立卓越中心。

This subproject is one of many research subprojects utilizing the resources provided by a Shared Instrumentation Grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the grant, which is not necessarily the institution for the investigator. DESCRIPTION (provided by applicant): (REVISED ABSTRACT) Funds are requested for a Finnigan Element2 magnetic sector field high-resolution inductively coupled plasma mass spectrometer (HR-ICP/MS). The Element2 is the most sensitive analytical instrument available for high-resolution multi-elemental/isotopic identification and quantitation. The requested instrument will serve as a state-of-the-art bioanalytical core resource for a diverse network of highly productive, innovative biomedical researchers at Harvard Medical School (HMS)/Harvard School of Public Health (HSPH) who require trace/ultra-trace analytical capabilities for a wide range of biomedical, metallomic, and metalloprotein biochemistry applications. Magnetic sector field technology supercedes quadrupole-based systems and provides unequivocal mass resolution, multi-elemental/isotopic sensitivity, polyatomic interference-free detection, and high-throughput performance and is the accepted standard for high resolution multi-elemental/isotopic applications in geochemistry, material science, and the semiconductor industry. Utilization of this technology in biomedical research is just beginning and allows metallomic assays, isotopic ratio dilution/tracer experiments, clinical toxicology/biomarker epidemiology analyses that have not been previously achievable. Very few HR-ICP/MS instruments are dedicated to biomedical/metallomic research anywhere in the world and none in the US. The requested instrument will be utilized as an inter-institutional cooperative core resource located at the Brigham & Women's Hospital, Boston. The instrument will be operated by an analytical team with over two decades of experience/expertise in biomedical trace metal analysis, including ICP/MS. The facility will be dedicated to biomedical metallomic research within the HMS/HSPH system and will be available to researchers at other regional institutions. Instrument siting capitalizes on proximity to major core facilities including the Trace Metal Laboratbry (HSPH), Harvard Institute of Medicine (HMS), Harvard Center for Genetics & Genomics, Harvard Center for Neurological Diseases, the Center for Aging, Genetics and Neurodegeneration, and the Taplin Biological Mass Spectrometry Facility (HMS). The Major Users Group includes interdisciplinary teams at the forefront of research in cellular/molecular biology, metalloprotein biochemistry, physiology, environmental toxicology and epidemiology, biometal metabolism/homeostasis, ophthalmology, urology, gastroenterology, and neuropsychiatry. Acquisition of this instrument will propel cutting-edge research in Alzheimer's disease, inflammatory and neoplastic diseases, cataracts, neurodevelopment, hypertension, and environmental toxicology. The principal investigator and Advisory Committee will promote equitable instrument access and cost-effective utilization to researchers throughout the geographic region. We anticipate that this core facility resource will catalyze discovery in biomedical metallomics and accelerate establishment of a Center of Excellence at the forefront of this new field.