Femtosecond Infrared Laser Ablation Platform

描述（由申请人提供）：申请资金用于飞秒红外激光消融（FIRLA）平台，用于耦合（连字符）到位于波士顿大学生物金属和金属组学中心的NCRR资助的高分辨率扇形场ICP质谱仪（SF-ICP-MS）。FIRLA-SF-ICP-MS联用系统将使代表整个区域和地球仪领先机构的40多个创新研究小组能够进行前所未有的超痕量元素和同位素绘图研究。该系统将成为全国首个此类专用生物金属资源。飞秒激光烧蚀技术提供了前所未有的空间分辨率，极大地提高了分析精度和精确度，并将附带的热损伤降至最低，从而首次以微米分辨率在生物标本中进行超痕量元素和同位素绘图研究。所请求的共享资源将促进波士顿大学医学院、波士顿大学公共卫生学院、哈佛医学院、哈佛公共卫生学院、马萨诸塞州总医院、布里格姆妇女医院、美国地质局、武装部队放射生物学研究所、劳伦斯伯克利国家实验室、爱尔兰博物馆、女王大学和墨尔本大学。在NCRR支持的生物金属和金属组学中心部署拟议的联用仪器将加速阿尔茨海默病，帕金森病，创伤性脑损伤，眼部疾病，环境毒理学，癌症生物学，微量营养素生理学，败血症和炎症，生物金属组学骨考古学，法医病理学，军事医学和纳米治疗学的前沿发现。所请求的仪器将作为与波士顿大学其他区域资源联合的共享资源进行管理，包括NCRR质谱资源、NIA阿尔茨海默病中心、NCRR临床和转化科学研究所、弗雷明汉研究和波士顿大学光子学中心。CBM由一个专家分析团队组成，该团队在生物医学金属组学分析、激光消融、激光诱导击穿光谱、元素和同位素ICP-MS以及光学发射光谱方面拥有20多年的经验。首席调查员和咨询委员会将促进公平获取和以具有成本效益的方式利用这一独特的分析资源。这种共享的工具和相关的核心资源将促进建立国家金属组学卓越中心，并在这一重要的新领域的前沿促进科学发现。 公共卫生相关性：申请资金用于在NCRR资助的波士顿大学生物金属和金属组学中心进行金属组织绘图的超快激光器。这一共享资源将支持阿尔茨海默病、帕金森病、创伤性脑损伤、肺癌、环境毒理学和纳米医学的开创性研究。

DESCRIPTION (provided by applicant): Funds are requested for a femtosecond infrared laser ablation (FIRLA) platform for coupling (hyphenation) to the NCRR-funded high-resolution sector field ICP mass spectrometer (SF-ICP-MS) located at the Center for Biometals and Metallomics at Boston University. The hyphenated FIRLA-SF-ICP-MS system will enable unprecedented ultra-trace elemental and isotopic mapping studies conducted by over forty innovative research teams representing leading institutions throughout the region and across the globe. The hyphenated system will be the first such dedicated biometallomic resource of its kind in the nation. Femtosecond laser ablation technology provides unprecedented spatial resolution with greatly improved analytical accuracy and precision with minimal collateral thermal damage, thus affording for the first time ultra-trace elemental and isotopic mapping studies in biological specimens at micron resolution. The requested shared resource will facilitate a wide range of innovative research conducted by leading interdisciplinary teams at Boston University School of Medicine, Boston University School of Public Health, Harvard Medical School, Harvard School of Public Health, Massachusetts General Hospital, Brigham & Women's Hospital, United States Geological Service, Armed Forces Radiobiology Research Institute, Lawrence Berkeley National Laboratory, Museum of Ireland, Queen's University, and the University of Melbourne. Deployment of the proposed hyphenated instrument at the NCRR-supported Center for Biometals and Metallomics will accelerate lead-edge discovery in Alzheimer's disease, Parkinson's disease, traumatic brain injury, ocular disorders, environmental toxicology, cancer biology, micronutrient physiology, sepsis and inflammation, biometallomic osteoarcheology, forensic pathology, military medicine, and nanotherapeutics. The requested instrument will be managed as a shared resource allied to other regional resources at Boston University, including the NCRR Mass Spectrometry Resource, NIA Alzheimer's Disease Center, NCRR Clinical and Translational Science Institute, the Framingham Study, and the Boston University Photonics Center. The CBM is staffed by an expert analytical team with over two decades of experience in biomedical metallomic analyses, laser ablation, laser-induced breakdown spectroscopy, elemental and isotopic ICP-MS, and optical emission spectroscopy. The Principal Investigator and Advisory Committee will promote equitable access and cost-effective utilization of this unique analytical resource. This shared instrument and allied core resource will facilitate establishment of a National Center of Excellence in metallomics and catalyze scientific discovery at the forefront of this important new field. PUBLIC HEALTH RELEVANCE: Funds are requested for an ultra-fast laser for metallomic tissue mapping at the NCRR-funded Center for Biometals & Metallomics, Boston University. This shared resource will support pioneering research in Alzheimer's disease, Parkinson's disease, traumatic brain injury, lung cancer, environmental toxicology, and nanomedicine.