CORE-- MASS SPECTROMETRY FACILITY

质谱核的开发和实现是上市公司研究的重要内容 首席调查员。特别是，岩心将使用拟议的自下而上提供质谱分析 以及采用Q-TOF技术和FTICR高分辨率分析的自上而下方法。 将使用自上而下(完整的蛋白质识别)和自下而上(蛋白质消化)的方法来 鉴定各种核糖体复合体的蛋白质。翻译后修改的差异将是 对于已经受到各种病毒(丙型肝炎， 登革热等)。将对与核糖体相关的eIF3因子进行分析和调查，以确定是否存在 磷酸化。既有现有FTICR的高分辨率能力，又有敏感度和灵活性 Q-TOF，结合成熟的样品制备方法，如IMAC，将允许 明确确定蛋白质成分和相关的翻译后修饰。

The development and implementation of the mass spectrometry core is essential to the research of the listed principal investigators. In particular the core will provide mass spectral analysis using the proposed bottomup and top-down methods employing both Q-TOF technology as well as FTICR high-resolution analysis. Both a top-down (intact protein identification) and a bottom-up (protein digestion) approach will be used to identify proteins of the various ribosomal complexes. Differences in post-translational modifications will be determined for ribosome complexes which have been subjected to infection by various viruses (hepatitis c, dengue, etc). eif3 factors associated with the ribosome will be analyzed and investigated for the presence of phosphorylation. Both the high-resolution capabilities of the exisiting FTICR and the sensitivity and flexibility of the Q-TOF, in combination with well established sample preparation methods such as IMAC, will allow for the unambiguous determination of protein constituents and associated post-translational modifications.