Core--Genomics

基因组学核心，在雅各布博士的指导下，将服务于考利，罗曼和格林的基因分型项目。 同源动物的基因组序列分析、测序，并为所有项目和核心提供生物信息学支持。这 Core生成与大鼠基因分型相关的所有湿实验室基因组数据，以生成同类基因和 使用简单序列长度多态性（SSLP）的亚同源动物，SSLP标记的产生， 克隆、测序、单核苷酸多态性（SNP）检测和物理作图（如果需要）。博士 Jacob在过去16年的研究中，在这个核心的各个方面都有丰富的经验。雅各布博士 他是威斯康星州医学院人类和分子遗传学中心（HMGC）主任。这 中心拥有所有的人员、设备、机器人和实验室信息管理系统 以满足本PPG的需求。基因分型组件，高度自动化，是为了使早期 繁殖决策，这使我们能够最大限度地减少老鼠住房的成本。基因组学核心还将指导 在Roman和格林的项目中，许多活动与突变检测和基因鉴定有关。最后，吉恩 狩猎、高通量测序和比较基因组学都需要重要的生物信息学 基础设施演进Jacob博士是大鼠基因组数据库（RGD）的PI，并指导生物信息学 在MCW的节目。因此，数据分析、基因鉴定和 预测和基因表达分析都已到位并可操作。

The Genomics Core, under the direction of Dr. Jacob, will serve Projects by Cowley, Roman, and Greene in the genotyping of the congenic animals, sequencing, and providing bioinformatics support for all the Projects and Cores. This Core generates all wet lab genomic data related to genotyping the rats to generate the congenic and subcongenic animals using simple sequence length polymorphisms (SSLPs), generation of SSLP markers, cloning, sequencing, single nucleotide polymorphism (SNP) detection and physical mapping (if needed). Dr. Jacob has extensive experience in all facets of this Core through his last 16 years of research. Dr. Jacob directs the Human and Molecular Genetics Center (HMGC) at the Medical College of Wisconsin (MCW). This center has in place all the personnel, equipment, robotics and laboratory information management systems required to meet the needs of this PPG. The genotyping component, highly automated, is set up to make early breeding decisions, which allows us to minimize the cost of rat housing. The Genomics Core will also direct many of the activities related to mutation detection and gene identification in Projects by Roman and Greene. Finally, gene hunting, high through-put sequencing and comparative genomics all require significant bioinformatics infrastructure. Dr. Jacob is the PI for the Rat Genome Database (RGD) and directs the Bioinformatics Program at MCW. As a result the analytical tools necessary for data analysis, gene identification and prediction, and gene expression analysis are all in place and operational.