Ribosomal glycopeptide production to investigate glycosylation changes in prostate cancer

蛋白质糖基化的变化是疾病的标志，但这些过程的潜在分子机制尚未完全了解。该项目将使用合成化学产生一系列糖基化氨基酸，这些糖基化氨基酸将与肽mRNA展示相结合，以产生糖肽展示方法，以完全覆盖蛋白质序列空间。这将用于鉴定疾病相关酶的底物，例如糖基转移酶GALNT7，其在前列腺癌中上调并驱动肿瘤生长。

Changes in glycosylation of proteins are hallmarks of disease, but the underlying molecular mechanisms of these processes are not fully understood. This project will use synthetic chemistry to produce a range of glycosylated amino acids that will be combined with peptide mRNA-display to generate a glycopeptide display method to completely cover protein sequence space. This will be used to identify the substrates of disease related enzymes such as the glycosyltransferase GALNT7, which is upregulated and drives tumour growth in prostate cancer.