HIV Vaccines via 2G12 Carbohydrate Epitope Mimicry

描述(申请人提供)：合成HIV碳水化合物表位疫苗。疫苗设计是基于最近关于人类抗体2G12与HIV蛋白gp120表面发现的寡糖结合作用的结晶学信息。本研究中的发现表明，2G12与几个糖链簇之间的多价相互作用的协同作用使2G12-gp120的强结合成为可能。在该实验室合成的疫苗将试图模拟两个或更多多糖的这种空间呈现，以形成类似的簇。在初步设计完成后，疫苗将与生物学家合作进行测试，看看它是否能刺激产生类似2G12的抗体，从而能够中和广泛的HIV-1分离株。根据这些研究的结果，疫苗将进行重新设计、重新合成和重新测试。这一过程的许多周期有望教会我们许多关于疫苗、艾滋病毒和有机合成的知识。

DESCRIPTION (provided by applicant): The synthesis of an HIV carbohydrate epitope vaccine is proposed. The vaccine design is based upon recent crystallographic information about the binding interactions of human antibody 2G12 with the oligosaccharides found on the surface of the HIV protein gp120. The findings in this study suggest that the strong 2G12-gp120 binding is made possible by the cooperativity of a multivalent interaction between 2G12 and a cluster of several glycans. The vaccine synthesized in this laboratory will seek to mimic this spatial presentation of two or more glycans to form a similar cluster. After the initial design has been synthesized, the vaccine will then be tested in collaboration with biologists, to see whether it stimulates the production of 2G12-like antibodies, capable of neutralizing a broad range of HIV-1 isolates. Based on the results of these studies, the vaccine will be redesigned, resynthesized and retested. Many cycles of this process will hopefully teach us a great deal about vaccines, HIV, and organic synthesis.