iCyt Reflection Cell Sorter

描述（由申请人提供）：本NCRR共享仪器拨款申请的目的是确保为弗吉尼亚大学医学院流式细胞术核心设施（FCCF）购买Reflection高速平行细胞分选仪的资金。FCCF是弗吉尼亚大学一个使用非常频繁的核心设施，它以服务收费的方式向大学社区提供与流式细胞术相关的服务，特别是向NIH资助的医学院研究人员提供服务。FCCF目前为来自27个不同部门的95个pi实验室的200多名研究人员提供服务。其中42个pi使用FCCF进行细胞分选。FCCF有一个带有DIVA选项的facadvantage SE涡轮分选机，目前该分选机接近满负荷运行，是UVA 70英里范围内唯一的电池分选机。自2001年FCCF重组以来，细胞分选服务的使用稳步增加，并在过去两年中呈爆炸式增长。由于FCCF设立的教育项目极大地提高了研究人员对流式细胞术和细胞分选的认识，同时也增加了医学院新教师的聘用，FCCF的使用得到了增加。目前的细胞分选器是提前两到四周预订的，这对依赖于细胞分选的正在进行的研究项目构成了严重的障碍。由于新细胞分选机的成本相对较高，阻碍了FCCF扩大规模以满足UVA社区的需求，因此FCCF的细胞分选服务受到了阻碍。购买和实施新的细胞分选机将1)允许FCCF满足当前对细胞分选的需求，2)满足在不久的将来增加容量的预期需求，3)在当前机器需要维修时提供备份能力，4)通过增加可用于分选的参数来增加UVA社区可用的服务。

DESCRIPTION (provided by applicant): The purpose of this NCRR Shared Instrument Grant application is to secure funding to purchase a Reflection" high speed parallel cell sorter for the Flow Cytometry Core Facility (FCCF) at the University of Virginia, School of Medicine. The FCCF is a very heavily used core facility at the University of Virginia and provides flow cytometry related services on a fee for service basis to the University community in general and particularly to NIH funded investigators in the School of Medicine. The FCCF currently serves over 200 investigators from the laboratories of 95 PIs from 27 different departments. Forty two of these PIs use the FCCF for cell sorting. The FCCF has a single FACSVantage SE Turbo sorter with DIVA option, which is currently run at near full capacity and is the only cell sorter within 70 miles of UVA. The use of cell sorting services has steadily increased since reorganization of the FCCF in 2001 and has exploded over the past 2 years. Increased use of the FCCF is the result of educational programs instituted by the FCCF that have greatly increased the awareness of investigators about flow cytometry and cell sorting as well as new faculty hires in the School of Medicine. The current cell sorter is booked two to four weeks in advance, which presents a serious impediment to ongoing research projects that are dependant on cell sorting. The cell sorting service in the FCCF has been hampered by the relatively high cost of a new cell sorter, which has prevented the FCCF from expanding to meet the needs of the UVA community. Purchase and implementation of the new cell sorter will 1) allow the FCCF to meet current demand for cell sorting, 2) meet anticipated requirements for increased capacity in the near future, 3) provide back up capability when the current machine needs repairs and 4) increase services available to the UVA community by increasing the parameters available for sorting.