Acquisition of an Electrospray Fourier Transform Mass Spectrometer

描述（由申请人提供）：斯克里普斯研究所质谱中心正在寻求资金，用于购买7.0 T电喷雾傅里叶变换质谱仪（FTMS），用于高分辨率/超高精度质量分析（<1-2 ppm）和高分辨率串联质谱实验（MSn，n=2-4）。所要求的FTMS仪器将是对我们现有的4.7T IonSpec MALDI FTMS的升级。这台MALDI FTMS仪器由Scripps公司在十年前购买，已经进行了超过50，000次分析，并被800多名研究人员使用。该中心急需这种高分辨率的能力，其NIH资助的研究人员。现有的FTMS仅配备了MALDI源，并且作为原始设计之一，在分析不同类别的化合物方面具有非常有限的灵敏度和通用性。该中心于2003年获得NIH资助，用于Agilent ESI TOF质谱仪，该质谱仪用于常规ESI-MS分析，并生成了超过45，000种化合物的数据。然而，该仪器和Scripps的所有其他质谱仪不提供ESI-FTMS所提供的高分辨率（> 100，000）、超高质量精度和高分辨率串联MS（MSn，n=2-4）能力。ESI FTMS将促进天然产物，代谢物，碳水化合物，肽，蛋白质，蛋白质翻译后修饰，寡核苷酸和所列研究人员和整个研究所其他实验室所需的合成有机化合物的表征。超过15名教师的研究将大大受益于7.0T升级的ESI FTMS功能。所有这些研究人员都得到了NIH的资助。其中两名研究人员，丽莎斯托尔斯和盛丁，是初级斯克里普斯教师谁也是最近接受国家卫生研究院的资金。这些群体包括200多名博士后和研究生，还提供了8个小用户（均由NIH资助）的表格。斯克里普斯认识到质谱和FTMS的重要性，目前斯克里普斯质谱中心雇用了8名全职员工来管理质谱，并承诺每年为该中心提供超过50万美元的资金。工作人员包括助理主任Sunia Trauger博士。他在布鲁克公司担任应用科学家，拥有7年的FTMS经验。此外，该机构已经承诺通过建造一个新的FTMS室（2001年），为MALDI-FTMS购买YAG激光器（2004年），以及可以与升级的7.0T ESI-FTMS一起使用的专用毛细管LC系统（2005年），投入超过30万美元。7.0 ESI-FTMS是生物分子鉴定和表征所需的重要工具。所要求的仪器将为我们的研究所带来新的能力，这些研究人员正在努力识别与癌症，病毒，囊性纤维化和干细胞的基础生物化学或治疗直接相关的分子。

DESCRIPTION (provided by applicant): The Center for Mass Spectrometry at The Scripps Research Institute is seeking funds for the purchase of a 7.0 T Electrospray Fourier transform mass spectrometer (FTMS) for high resolution/ultra-high accuracy mass analysis (<1-2 ppm) and high resolution tandem mass spectrometry experiments (MSn, n=2-4). The requested FTMS instrumentation would be an upgrade to our existing 4.7T IonSpec MALDI FTMS. This MALDI FTMS instrument, purchased by Scripps ten years ago, has performed over 50,000 analyses and used by over 800 researchers. The Center is in critical need of this high resolution capability for its NIH funded researchers. The existing FTMS is equipped only with a MALDI source and as one of the original designs, has very limited sensitivity and versatility with respect to analyzing different classes of compounds. The Center received NIH funding in 2003 for an Agilent ESI TOF mass spectrometer, which is being used for routine ESI-MS analyses and has generated data on over 45,000 compounds. However this instrument and all other mass spectrometers at Scripps do not offer the high resolution (>100,000), ultra-high mass accuracy and high resolution tandem MS (MSn, n=2-4) capabilities that the ESI-FTMS would offer. The ESI FTMS would facilitate the characterization of natural products, metabolites, carbohydrates, peptides, proteins, protein post-translational modifications, oligonucleotides and a host of synthetic organic compounds required by the researchers listed and other laboratories throughout the institute. The research of over fifteen faculty members would greatly benefit from ESI FTMS capabilities of the 7.0T upgrade. All of these researchers have NIH funding. Two of the investigators, Lisa Stowers and Sheng Ding, are junior Scripps faculty who are also recent recipients of NIH funding. These groups include over 200 postdoctoral associates and graduate students, a table of eight minor users (all NIH funded) is also provided. Scripps recognizes the importance of mass spectrometry and FTMS with the current employment of eight FTEs to manage mass spectrometry at the Scripps Center for Mass Spectrometry with a financial commitment to staff the Center of over $500,000 per year. The staff includes Assistant Director Sunia Trauger, Ph.D. who has 7 years of experience with FTMS at Bruker as an Applications Scientist. In addition, the instituted has already committed over $300K through the construction of a new FTMS room (2001), the purchase of a YAG laser for the MALDI-FTMS (2004), as well as a dedicated capillary LC system (2005) that can be used with the upgraded 7.0T ESI- FTMS. The 7.0 ESI-FTMS is an essential tool needed for the identification and characterization of bio-molecules. The requested instrument will bring new capabilities to our institute for a diverse group of researchers who are working towards identifying molecules that are directly related to the fundamental biochemistry or treatment of cancer, viruses, cystic fibrosis and stem cells.