Bioscope II - AFM system for Nanoimaging Core Facility

描述（由申请人提供）：内布拉斯加州大学医学中心（UNMC）正在申请一个由Bioscope II原子力显微镜（AFM）和倒置Olympus IX71光学显微镜组成的集成系统，用于其纳米成像核心设施。所要求的仪器将由最先进的AFM显微镜组成，能够成像和探测从单分子到活细胞的各种尺寸的样品。Bioscope II支持所有AFM功能，包括在空气和水溶液中成像，并配备了允许活细胞分析的模块。Bioscope II设计用于集成到倒置显微镜中，允许倒置显微镜的高分辨率亮场和荧光成像能力。所提出的设计能够使用集成系统的两个成像模块对同一样品进行并行和同时分析。所选的奥林巴斯IX71倒置显微镜将配备物镜-全内反射荧光分析模块。所要求的高速和高分辨率C9100-12 EM CCD相机允许单分子荧光检测与毫秒级的时间分辨率。离线数据分析工作站将装载AFM和光学显微镜数据分析所需的软件。所要求的仪器将安装在多用户纳米成像核心设施中，并将由训练有素的人员仔细监督。该仪器将提供给来自UNMC的10名NIH资助的调查人员。这些项目包括研究基因组完整性和与癌症发展相关的过程的分子机制（Eppley癌症中心），开发药物设计和输送的新方法（药学院和药物输送和纳米医学中心），以及理解神经退行性疾病的发展机制（神经生物学和神经退行性疾病中心）。事实上，该仪器的潜在用户数量相当大，到目前为止，已有30多名研究人员对所要求的集成AFM/光学显微镜系统表达了浓厚的兴趣。来自当地社区的研究人员对新型AFM/IX71仪器的使用非常感兴趣，来自克莱顿大学医学中心的NIH资助的研究人员是当前仪器的主要用户之一。校园外有两位NIH资助的研究人员也想使用集成Bioscope/IX71系统的独特功能。

DESCRIPTION (provided by applicant): The University of Nebraska Medical Center (UNMC) is requesting an integrated system consisting of the Bioscope II Atomic Force Microscope (AFM) and an inverted Olympus IX71 optical microscope for its Nanoimaging Core Facility. The requested instrument will be comprised of the state of the art AFM microscope capable imaging and probing the samples with a broad range of sizes spanning from single molecules to live cells. The Bioscope II supports all AFM capabilities including imaging in air and aqueous solutions and equipped with the module allowing analysis of live cells. The Bioscope II is designed for the integration into an inverted microscope allowing the high resolution bright field and fluorescence imaging capabilities of the inverted microscope. The proposed design is capable of the parallel and simultaneous analysis of the same sample with both imaging modules of the integrated system. The selected Olympus IX71 inverted microscope will be equipped with the module for the objective-through total internal reflection fluorescence analysis. The requested high speed and high resolution C9100-12 EM CCD camera allows the single molecule fluorescence detection with the millisecond temporal resolution. The off-line data analysis workstation will be loaded with the software requited for analysis of the AFM and optical microscopy data. The requested instrumentation will be installed in the multi-user Nanoimaging Core Facility and will be carefully overseen by the trained personnel. This instrument will be available to the pool of 10 NIH funded investigators from the UNMC. The projects include studies of molecular mechanisms of processes responsible for the integrity of the genome and related to the cancer development (the Eppley Cancer Center), development of novel approaches for the drug design and delivery (College of Pharmacy and the Center for Drug Delivery and Nanomedicine) and understanding the mechanisms of development of neurodegenerative diseases (the Center for Neurobiology and Neurodegenerative Disorders). In fact, the pool of potential users of the instrumentation is considerably larger, so far more than 30 investigators were expressed their strong interest in the requested integrated AFM/optical microscope system. The researchers from local community are very interested in the use of the novel AFM/IX71 instrumentation, and the NIH funded investigator from the Creighton University Medical Center is one of the current instrument major users. There are two NIH funded investigators outside the campus who also want to use the unique features of the integrated Bioscope/IX71 system.