Exposure to Bisphenol A & Reproductive Effect in Humans

描述(申请人提供)：双酚A(BPA)被强烈怀疑为人类内分泌干扰物。我们建议跟进我们早些时候发表的关于双酚A对中国四家化工厂工人的人类生殖毒性的初步报告。我们预计将招募(A)300名现任和前任接触双酚A的工人(200名男性和100名女性)，(B)300名配偶，用于检查中等接触水平，以及(C)300名子女，用于检查多代影响。对于每名男性工人，将从当地纺织厂选择两名没有接触双酚A历史的对照组。由于接触女工人数较少，每名接触女工将选择4个对照，因此将招募800名对照及其配偶和子女。我们的具体目标如下：(1)在女性(包括接触工人和接触男性工人的配偶)中，接触是否与性激素谱、月经紊乱、性交频率、怀孕时间和自然流产有关？(2)在男性(包括接触者和接触女工的配偶)中，接触是否与性激素谱、抑制素B水平、精液质量和性交频率有关？(3)在接触者的子女中，父母接触是否与性别比、出生体重、第二性征发育年龄、月经特征、月经特征、性激素水平呢？目前的双酚A接触将通过个人监测从空气样本中获得。累积风险暴露将通过半定量的工作/风险暴露矩阵进行估计。将进行面谈，以获得有关工作历史、生殖和性史以及子女青春期年龄的详细信息。血液和精液样本将被评估激素水平和精液质量。这项工作将由上海市计划生育研究所和复旦大学(原上海医科大学)职业健康系与复旦大学合作进行 华盛顿的。他们在将在研究中使用的数据收集和实验室分析方法方面拥有丰富的经验。这项拟议的研究将评估双酚A在高水平暴露环境中的生殖影响。这项研究还将调查对多代人的影响。

DESCRIPTION (provided by applicant): Bisphenol A (BPA) is strongly suspected as a human endocrine disrupter. We propose to follow up our earlier published preliminary report on the human reproductive toxicity of BPA among workers in four Chinese chemical plants. We expect to recruit (a) 300 current and former BPA-exposed workers (200 men and 100 women), (b) 300 spouses, for examining intermediate exposure levels, and (c) 300 offspring, for examining multigenerational effects. For each male worker, two controls with no history of exposure to BPA will be selected from local textile factories. Because of the smaller number of exposed female workers, four controls will be selected per exposed female worker, thus 800 controls with their spouses and offspring will be recruited. Our specific aims are as follows: (1) Among women (both exposed workers and spouses of male exposed workers), is there a relationship of exposure with sex hormone profile, menstrual disorders, frequency of sexual intercourse, time-to pregnancy, and spontaneous abortion? (2) Among men (both exposed workers and spouses of female exposed workers), is there a relationship of exposure with sex hormone profile, inhibin B levels, semen quality, and frequency of sexual intercourse? (3) Among the children of exposed workers, is there a relationship of parental exposure with sex ratio, birth weight, age at development of secondary sex features, menstrual characteristics, and sex hormone profile? Current BPA exposure will be obtained from air sampling through personal monitoring. Cumulative exposure will be estimated through a semi-quantitative job/exposure matrix. An in-person interview will be conducted to obtain detailed information on work history, reproductive and sexual history, and the age at puberty of offspring. Blood and semen samples will be evaluated for hormone levels and semen quality. The work will be conducted by the Shanghai Institute of Planned Parenthood Research and Fudan University (formerly Shanghai Medical University), Department of Occupational Health in collaboration with University of Washington. They have extensive experience in the data collection and lab analysis methods that will be used in the study. The proposed study will evaluate the reproductive effects of BPA in settings where there have been high levels of exposure. The study will also investigate effects in multiple human generations.