Novel Multimodal Selectors for CE and HPLC

描述（申请人提供）：目前，高效液相色谱分离发展最快的领域是生物分子分离和液相色谱-质谱（LC-MS）领域。虽然在过去的几十年里，质谱法取得了惊人的进步，但与质谱仪相结合的分离固有的缺陷仍然给获取生物样品中的全部信息内容带来了相当大的障碍。生物分子分离具有挑战性，因为它们呈现出从疏水到极性到静电的各种相互作用模式。提出的工作解决了对生物聚合物（例如，多肽，蛋白质，寡核苷酸）的新颖和更通用的分离策略改进技术的迫切需求，同时使溶质/离子液体相互作用的表征成为可能。本研究的目标是将离子液体这一新兴领域整合到生物分子分离的新分离策略中，以提供一种快速评估离子液体/目标分析物相互作用的方法，以及一种能够分离复杂生物分子混合物的新型固定相。

DESCRIPTION (provided by applicant): Currently, the fastest growing areas of high performance liquid chromatographic separations are in the areas of biomolecule separations and liquid chromatography-mass spectrometry (LC-MS). While the last few decades have seen phenomenal progress in mass spectrometry, the shortfalls inherent in the separations coupled to the mass spectrometer still present considerable barriers to accessing the full information content in biological samples. Biomolecule separations are challenging because they present a variety of interaction modalities ranging from hydrophobic to polar to electrostatic. The proposed work addresses the urgent need for improved technologies for novel and more universal separation strategies for biopolymers (e.g., polypeptides, proteins, oligonucleotides) while simultaneously enabling characterization of solute/ionic liquid interactions. The objective of the proposed work is to integrate the emerging field of ionic liquids into novel separation strategies for biomolecular separations with the goal of providing a rapid method for assessing ionic liquids/target analyte interactions a new class of stationary phases enabling separations of complex biomolecular mixtures. We hypothesize that the proposed selectors will provide multimodal retention for the separation of a wide variety of biomolecular analytes. Two specific aims will address this hypothesis: 1. CE Studies of Ionic Liquid Selectors. Capillary electrophoresis will be used to rapidly screen for ionic liquids/selected analyte interactions. We will relate migration data to ionic liquid/analyte affinity. 2. LC Studies of Ionic Liquid Selectors. Retention data will be obtained on the proposed HPLC sorbents beginning systematically with small molecules (e.g., aromatics, nucleotides, amino acids) and extending to larger molecules (e.g., peptides, polynucleotides) as the separation space encompassed by this new media is mapped out.