Tracking immune cell migration in tumour and normal tissues after combined radiation-drug therapy in Kaede mice

凯德小鼠被转基因了一种来自石珊瑚TrachyPhyllia geoffroyi的可光激活的荧光蛋白。体内紫外光照射后，光场中的细胞不可逆地从绿色荧光转变为红色荧光。候选人将使用Kaeed小鼠来跟踪免疫细胞在照射和未照射组织中的迁移和激活/抑制(通过流式细胞仪、荧光显微镜、转录图谱)。还将评估其他免疫调节疗法的效果，包括化疗、DNA损伤反应(DDR)和免疫检查点抑制剂。候选人将分析剂量分割方案、淋巴结照射或屏蔽以及空间剂量调制对免疫细胞运输的影响。

Kaede mice are transgenic for a photoactivatable fluorescent protein derived from a stony coral, Trachyphyllia geoffroyi. Following in vivo ultraviolet illumination, cells in the light field irreversibly photoconvert from green to red fluorescence. The candidate will use Kaede mice to track migration and activation/suppression of immune cells (by flow cytometry, fluorescence microscopy, transcriptional profiling) in irradiated and unirradiated tissues. The effects of additional immunomodulatory therapies, including chemotherapy, DNA damage response (DDR) and immune checkpoint inhibitors, will also be assessed. The candidate will analyse the effects of dose-fractionation regimens, lymph node irradiation or shielding and spatial dose-modulation on immune cell trafficking.