Commercialization of Embryonic Kidney Cell Lines

描述（由申请人提供）：迫切需要增加肾脏疾病研究的资源和人力。Probetex成立时是一家“肾脏研究资源公司”，旨在创建一个“现成”组织产品库，提供现成的样本，并帮助研究人员快速有效地探索他们对已建立和标准化模型或已确定的人类疾病病例的想法。这一目标的一部分是建立一个肾细胞系库，以补充其肾组织产品组合。事实上，肾祖细胞的分离、克隆、维持和表征在国家糖尿病、消化和肾脏疾病研究所和美国肾脏学会理事会主办的战略规划会议报告中被确定为研究重点。这些优先事项包括：1)开发新的器官型体外培养模型；2)开发允许干细胞进入肾祖细胞的条件，然后维持这些分化的细胞系。该I期申请（与位于圣安东尼奥的德克萨斯大学健康科学中心合作）的基础是通过定义来自小鼠和大鼠胚胎原基的三种现有肾脏祖细胞系的生长和分化条件，以及从发育中的胚胎肾脏中分离和表征新细胞系，来解决上述优先事项。具体目标是：目标1。目的探讨肾原基衍生的胚胎后肾间充质（MM）和输尿管芽（UB）细胞系的分化潜力。在SCID小鼠中，分化条件包括共培养、特殊条件培养基、细胞单独和联合植入后的生长。将使用免疫组织化学和RT-PCR进行表达分析，以验证扩展的一组特定细胞类型和细胞分化状态的表达标记。目标2。分离和鉴定来自胚胎后肾肾源区新的胚胎细胞系，并使用上述标准鉴定细胞；目标3和目标4旨在建立胚胎肾细胞库和商业化计划，以扩大新细胞系，大规模生产肾细胞繁殖协议，外包和许可，旨在扩大Probetex存储库中的细胞系范围，以供公司的研究者/客户使用。

DESCRIPTION (provided by applicant): There is a crucial need for increases in resources and manpower in research in kidney disease. Probetex was founded as a "Renal Research Resource Company" to create a repository of "off the-shelf" tissue products to provide readily available samples and help investigators to quickly and efficiently explore their ideas on established and standardized models or identified cases of human disease. Part of this goal is to create a repository of kidney cell lines to supplement its portfolio of renal tissue products. Indeed, the isolation, cloning, maintenance, and characterization of renal progenitor cells is identified as a research priority in a Report of the Strategic Planning Conferences sponsored by the National Institute of Diabetes and Digestive and Kidney Diseases and the Council of American Kidney Societies. Included in these priorities are initiatives to 1) develop new organotypic in vitro culture models and 2) develop conditions to permit commitment of stem cells into renal progenitors, and then the maintenance of these differentiated cell lines. The basis of this Phase I application (in collaboration with the University of Texas Health Science Center at San Antonio) is to address the above priorities by defining growth and differentiation conditions for three existing kidney progenitor cell lines derived from mouse and rat embryonic primordia and to isolate and characterize new cell lines from developing embryonic kidney. Specific aims are: Aim 1. To characterize the differentiation potential of embryonic metanephric mesenchymal (MM) and ureteric bud (UB) cell lines derived from kidney primordia. Differentiation conditions include growth in co-culture, in specialized conditioned media, and after implantation of the cells individually and in combination in SCID mice. Expression analysis will be conducted using immunohistochemistry and RT-PCR to validate expanded set of expression markers for specific cell types and differentiation states of the cells. Aim 2. To isolate and characterize new embryonic cell lines derived from the nephrogenic zone of embryonic metanephric kidney and characterize the cells using criteria as outlined above; Aims 3 and 4 are designed to establish an Embryonic Kidney Cell Bank and Commercialization Plan for expansion of new cell lines, large-scale production protocols for kidney cell propagation, out-sourcing, and licensing with the intent to expand the scope of cell lines in the Probetex's repository that are available to the company's investigator/ customers.