Design and evolution of metalloenzymes for efficient and selective CO2 reduction

该项目将创建从头酶，用于将CO2有效和选择性地转化为CO和商品化学品，基于PI实验室开发的第一代从头CO2还原酶。这些从头酶的表征将提供控制CO2还原和转化的活性位点特征和催化机制的详细理解，以支持未来几代催化剂的设计。这些目标将通过采用计算酶设计，结构生物学，遗传密码扩展，定向进化和生物物理酶表征的最先进的方法来实现。

This project will create de novo enzymes for efficient and selective conversion of CO2 into CO and commodity chemicals, building from a first generation de novo CO2 reductase enzymes developed in the PI lab. Characterization of these de novo enzymes will provide a detailed understanding of the active site features and catalytic mechanisms controlling CO2 reduction and transformation to underpin the design of future generations of catalysts. These ambitions will be achieved by employing state-of-the-art methods in computational enzyme design, structural biology, genetic code expansion, directed evolution and biophysical enzyme characterization.