Center for Women's Health and Reproduction

该大学生殖研究专业合作中心计划（SCCPRR）的目标是 该研究所将在生殖科学领域开展创新的基础和临床研究。提出了四个项目。 3 roplasty I和n关注子宫内膜异位症的病因学和病理生理学，而项目III和IV关注重要的 啮齿动物模型中有关蜕膜化和着床的问题。对细胞周期调控的认识， 然而，在这些啮齿动物模型中的三元共聚物和金属蛋白酶，将直接适用于理解 人类生殖生理学和病理学。项目I“狒狒子宫内膜异位症”-将检验这一假设 子宫内膜异位症的发展分为两个不同的阶段。生成的数据将确定卵巢是否 类固醇是必要的，首先建立疾病，如果当地类固醇生物合成将维持它。该项目还将 建立子宫内膜异位症和子宫容受性异常之间的直接联系。项目二：“激素在 子宫内膜异位症”-将检验子宫内膜异位组织中的孕酮抗性破坏子宫内膜异位症的诱导的假设。 雌二醇（E2）代谢酶17/？-羟脱氢酶（HSD）2型。一个主要目标是确定是否 核受体表达的异常引起孕酮抵抗和17 P-HSD-2的缺失引起孕酮抵抗。 增加的组织E2水平是子宫内膜异位症中细胞命运改变的原因。项目PI“细胞信号传导和基因 蜕膜发育的调节”将侧重于蜕膜衍生蛋白的功能作用。利用转基因 良好的模型，细胞周期抑制剂，激活素A和催乳素在发育和维持中的作用和调节 ）f将对妊娠期间的蜕膜进行评价。项目IV“EMMPRIN调节细胞中的金属蛋白酶 子宫内膜”检验了子宫内膜分泌的金属蛋白酶（EMMPRIN）诱导剂 上皮细胞调节子宫内膜中金属蛋白酶（MMP）的产生。这些研究将侧重于 MMP在着床中的重要性，并确定EMMPRIN表达是否是MMP产生所必需的， 子宫内膜组织。这些项目将得到一个行政核心和两个研究核心的支持： 显微镜检查和B）组织获取和细胞培养。这些研究核心将以开放获取模式运作 md支持其他7个NIH批准的赠款，其研究目标将有助于但不会侵犯 U 54申请伊利诺伊大学的U 54中心将建立在一个强大的互动和 collegia！环境和一个在生殖研究方面有着丰富历史的机构。

The objective of the Specialized Cooperative Centers Program in Reproductive Research (SCCPRR) at the University of llinois is to conduct innovative basic and clinical research in the reproductive sciences. Four projects are proposed. 3rojects I and n focus on the etiology and pathophysiology of endometriosis while Projects HI and IV address important questions relating to decidualization and implantation in rodent models. Insights gained on the regulation of cell cycle, ipoptosis and metalloproteinases in these rodent models, however, will be directly applicable to understanding the jhysiology and pathobiology of human reproduction. Project I "Endometriosis in the Baboon" - will test the hypothesis hat the development of endometriosis occurs in two distinct phases. The data generated will determine if ovarian steroids are necessary to first establish the disease and if local steroid biosynthesis will maintain it. This project will also establish a direct link between endometriosis and aberrations in uterine receptivity. Project II "Hormone Action in indometriosis" - will test the hypothesis that progesterone resistance in endometriotic tissue disrupts the induction of the cey estradiol (E2) metabolizing enzyme 17/?-hydroxydehydrogenase (HSD) type 2. A major goal is to determine whether iberrations in nuclear receptor expression causing progesterone resistance and the absence of 17P-HSD-2 giving rise to increased tissue E2 levels are responsible for altered cell fate in endometriosis. Project PI "Cell Signaling and Gene Regulation in Decidual Development" will focus on the functional role of decidual-derived proteins. Using transgenic nice models, the role and regulation of cell cycle inhibitors, activin A and prolactin in the development and maintenance )f the decidua during pregnancy will be evaluated. Project IV "EMMPRIN Regulates Metalloproteinases in the Endometrium" tests the hypothesis that an inducer of metalloproteinases (EMMPRIN) that is secreted by uterine epithelial cells regulates metalloproteinase (MMP) production in the endometrium. These studies will focus on the importance of MMPs in implantation and determine if EMMPRIN expression is required for MMP production by ;ndometriotic tissue. These projects will be supported by an administrative core and two research cores: A) Imaging and Microscopy and B) Tissue Procurement and Cell Culture. These research cores will operate in an open access formula md support 7 other NIH approved grants whose research objectives will contribute but will not infringe upon the goals of he U54 application. The U54 Center at the University of Illinois will be established within a strong interactive and collegia! environment and in an institution that has a rich history in reproductive research.