STEM CELL DERIVATION OF TWO NEOTROPICAL PRIMATE SPECIES

描述（由申请人提供）：这是一个SERCA，用于支持Dr. A.南亚拉巴马大学比较医学系的助理教授米歇尔舒勒说。舒勒博士获得学士学位。1993年，她在宾夕法尼亚州立大学的动物生物科学专业，1997年从奥本大学毕业，从ACLAM认可的博士后奖学金/博士2003年在伯明翰的亚拉巴马大学的分子和细胞病理学项目。本申请中描述的项目的总体目标是优化新热带灵长类动物的辅助生殖技术，最终目标是从这些物种中开发胚胎干细胞系。舒勒博士召集了一个由资深研究人员组成的小组（南亚拉巴马大学的乔纳森·斯卡梅尔博士和博特罗斯·里兹克博士; MD安德森癌症中心（巴斯特罗普，德克萨斯州）的Abee博士;俄勒冈州国家灵长类动物研究中心的Donald Wolf博士和玛丽Zelinski- Wooten博士;斯科特和白色医院（坦普尔，德克萨斯州）的托马斯Kuehl博士;埃默里大学（亚特兰大，佐治亚州）的Anthony Chan博士），他们为申请人制定了详细的培训计划。该项目的具体目标是：（1）确定松鼠猴和猫头鹰猴卵巢刺激和卵母细胞收集的最佳策略;（2）确定松鼠猴和猫头鹰猴胞质内精子注射（ICSI）和/或体外受精（IVF）的最佳方法，包括卵母细胞和胚胎的最佳培养技术;（3）优化子宫冲洗法回收囊胚的条件;（4）建立松鼠猴和枭猴胚胎干细胞系。该应用程序利用了美国新热带灵长类动物研究和资源中心（CNPRR）的独特环境，并对国家研究资源中心2004-2008年战略计划中概述的计划目标做出了响应。

DESCRIPTION (provided by applicant): This is a SERCA to support the scientific development of Dr. A. Michele Schuler, an Assistant Professor in the Department of Comparative Medicine at the University of South Alabama. Dr. Schuler received her B.S. in Animal Bioscience at Penn State University in 1993, her D.V.M. from Auburn University in 1997, and her Ph.D. from the ACLAM-accredited postdoctoral fellowship/Ph.D. program in Molecular and Cellular Pathology at the University of Alabama at Birmingham in 2003. The overall goal of the project described in this application is to optimize assisted reproductive technologies in neotropical primates with the ultimate goal of developing embryonic stem cell lines from these species. Dr. Schuler has assembled a panel of well established investigators (Dr. Jonathan Scammell and Dr. Botros Rizk at the University of South Alabama; Dr. Christian R. Abee at MD Anderson Cancer Center, Bastrop, TX; Dr. Donald Wolf and Dr. Mary Zelinski- Wooten at Oregon National Primate Research Center; Dr. Thomas Kuehl at Scott and White Hospital, Temple, TX; and Dr. Anthony Chan at Emory University, Atlanta, GA) who have developed a detailed training program for the applicant. The specific aims of the project are: (1) To determine the best strategy for ovarian stimulation and oocyte collection in squirrel monkeys and owl monkeys; (2) To determine the optimal method for intra-cytoplasmic sperm injection (ICSI) and/or in vitro fertilization (IVF) in squirrel and owl monkeys including optimal culture techniques for oocytes and embryos; (3) To optimize conditions for retrieval of in vivo-derived blastocysts by uterine flushing; (4) To develop embryonic stem cell lines from squirrel monkeys and owl monkeys. This application takes advantage of the unique environment of the Center for Neotropical Primate Research and Resources (CNPRR) at USA and is responsive to programmatic objectives outlined in the National Center for Research Resources 2004-2008 Strategic Plan.