Opioid Modulation of Cue-Triggered 'Wanting' in Amygdala

描述(由申请人提供)：这项建议的目的是探索杏仁核中的阿片受体(MORS)对自然奖赏相关线索的激励动机的归因。初步结果表明，向杏仁核注入特定的MOR激动剂DAMGO(0.1mgDAMGO)可以增加雌性大鼠的摄食行为。此外，我们发现，在自动变形训练的前六天中的每一天之前给予DAMGO都会增加对两个与奖励相关的线索之一的接近程度，这取决于大鼠在“首选”线索中的个体差异。在拟议的实验中，我们首先试图确定这些增强在CS+接近行为中的剂量依赖性和MOR中介作用。其次，我们试图确定观察到的CEA DAMGO的食欲效应是否是杏仁核特有的。第三，我们试图确定自我塑造前焦虑、探索性和奖赏敏感性的个体差异是否与观察到的自我塑造个体差异有关。这个项目与了解成瘾和肥胖等人类食欲障碍的神经基础和个体差异的作用有关。

DESCRIPTION (provided by applicant): The aim of this proposal is to explore the involvement of mu opioid receptors (MORs) in the amygdala on the attribution of incentive motivation to natural reward-associated cues. Preliminary results indicate that infusion of the specific MOR agonist DAMGO (0.1 mu g) into the amygdala increases feeding behavior in female rats. Additionally, we found that DAMGO administered prior to each of the first six days of autoshaping training increases approaches to one of two reward associated cues, depending on individual differences between rats in 'preferred' cue. In the proposed experiments, we first seek to determine the dose dependency and MOR mediation of these enhancements in CS+ approach behavior. Second, we seek to determine whether observed appetitive effects of CeA DAMGO are specific to the amygdala. Third, we seek to determine whether individual differences in pre-autoshaping anxiety, exploratory, and reward sensitivity are related to individual differences observed in autoshaping. This project is relevant to understanding the neural substrates of, and role of individual differences in human appetitive disorders such as addiction and obesity.