Modifying HA biomaterials with collagen-derived peptides

描述（由申请人提供）：由粘附分子内短序列组成的合成肽，称为“仿生肽”，已被用于促进细胞在许多不同类型的植入生物材料上的粘附。然而，我们的实验室已经证明羟基磷灰石（HA）生物材料具有从血液/血清中吸附细胞附着因子的能力，这使我们质疑仿生方法是否对这种生物材料有帮助。我们选择探索在血液中不存在高丰度的蛋白质的模拟肽的使用，但在成骨细胞前分化中发挥了重要作用。胶原蛋白是骨骼有机相的主要成分，但在血液中并不大量存在。结合胶原I的整合素受体的激活引起下游信号效应，导致成骨细胞标志物的增加。我们的目标是测试三种I型胶原模拟肽的功效，看看哪一种，如果有的话，有能力增加初始细胞粘附或分化率。

DESCRIPTION (provided by applicant): Synthetic peptides composed of short sequences within adhesion molecules, referred to as "biomimetic peptides", have been used to promote cell adhesion on many different types of implant biomaterials. However, our laboratory has shown that hydroxyapatite (HA) biomaterials have the ability to adsorb cell attachment factors from blood/serum, and this caused us to question whether biomimetic approaches-would be helpful on this biomaterial. We opted to explore the use of mimetic peptides of proteins which were not present in high abundance in the blood, but played a major roll in pre-osteoblast cell differentiation. Collagen is the major component of the organic phase of bone, but is not present in large quantities in the blood. Activation of the integrin receptors which bind collagen I cause downstream signaling effects which lead to an increase in osteoblastic markers. Our goal is to test the efficacy of three collagen I mimetic peptides, to see which, if any, has the ability to increase initial cell adhesion or the rate of differentiation.