Mechanisms Of Synaptic Plasticity In The Adult And Devel

环境健康的一个长期问题是需要了解环境在人类大脑发育中所起的作用。新生儿的大脑特别容易受到感官环境的破坏，这可能对其生理和形态产生深远的影响。发育中的大脑对感官操纵或环境毒物的环境影响的这种易感性在出生后生命的特定关键时期尤为明显。一方面，这种易感性使发育中的大脑特别容易受到有毒物质的伤害。另一方面，神经元或突触之间连接的可塑性，对于在出生后发育过程中完善大脑回路至关重要。类似的突触变化机制可能是成人学习的基础。因此，我们的主要兴趣是确定突触长期可塑性的分子基础。为了了解突触变化如何持续一生，我们研究了神经元活动如何调节基因转录以巩固突触变化。

A longstanding issue in environmental health is the need to understand the role the environment plays in human brain development. The brain of the neonate is particularly susceptible to disruption of the sensory environment, which can have profound effects on its physiology and morphology. Such susceptibility of the developing brain to environmental influence by sensory manipulation or to environmental toxicants is particularly pronounced during defined critical periods of postnatal life. On the one hand, this susceptibility makes the developing brain particularly vulnerable to toxic insults. On the other hand, the plasticity of the connections between neurons, or synapses, is critical for refining brain circuitry during postnatal development. Similar mechanisms for changing synapses are likely to serve the basis for learning in the adult. Our primary interest, therefore, has been to determine the molecular basis of long-lasting synaptic plasticity. To understand how synaptic changes persist for a lifetime, we study how neuronal activity regulates gene transcription to consolidate synaptic changes. Evidence suggests that the long-term changes in synaptic efficacy require expression of new RNA and toward that end, we have focused on the regulation of gene transcription by neuronal action potentials. One kinase that is known to be turned on with neuronal activity is the Extracellular signal Regulated Kinase (ERK). We use nuclei isolated from small amounts of brain tissue, which had first been electrically stimulated in vitro (in the case of slices) or treated with drugs to stimulate neuronal activity (in the case of neuronal cultures). In these preparations, we have identified a high molecular weight entity reactive to antibodies against active ERK that increases after neuronal stimulation. We have further identified putative components of this complex and hypothesize that the enzyme transglutaminase, which can crosslink proteins in a calcium dependent fashion, is important in stabilizing the nuclear complexes. This process may be important in anchoring ERK pathway components in the nucleus to facilitate or direct transcription. In a related study, we have established that blockade of the N-methyl-D-aspartate receptor inhibits ERK activation by blocking action potential generation induced with certain stimulation patterns. These results establish the importance of action potentials in ERK activation in response to synaptic activity. Ongoing studies in the lab are directed toward understanding ERK-dependent modulation of transcription factors that regulate genes induced with neuronal activity. These studies examining transcriptional regulation by neuronal activity will lead to a better understanding of how genes required for synaptic plasticity are regulated. Some insights into synaptic plasticity might be gained by comparing highly plastic brain areas, such as the hippocampus, with less plastic areas, such as layer 4 of the cerebral cortex. The hippocampus is critical for memory and spatial navigation. One area of the hippocampus, the CA2, however, shares with layer 4 expression of several of genes (TREK-1, for example). Interestingly, the CA2 has been noted for its resistance to disease and damage from trauma, ischemia, and stroke. We found that CA2 is similarly resistant to forms of synaptic plasticity including synapse strengthening (long-term potentiation) and synaptic weakening (long-term depression), even though synaptic responses in CA2 were very similar to those in the neighboring CA1 and CA3 areas. Because CA2 and its surrounding regions are anatomically very similar, these findings may therefore lead to identification of critical molecular components in the pathways leading to not only synaptic plasticity, but also neuronal damage and death. Our longer term interests are aimed at determining first, the nature of the developmental down-regulation of synaptic plasticity in the form of critical periods, and second, how neuronal activity leads to synapse elimination. We have begun to develop techniques by which activity-dependent synapse elimination during critical periods can be studied. By understanding the molecular and cellular mechanisms of synaptic plasticity during development, we may begin to understand how exposure to environmental toxicants during development can have life-long consequences on cognition and susceptibility to disease.