Translational Systems

翻译系统核心(核心C)将与项目负责人(Hammond、Insel和 蒙明尼)、A核心(费拉米斯科)和B核心(宫原)。此共享资源旨在提供计划 科学家，在一个单一的设施中，特定的技术是劳动密集型的，高度专业化，而不是一般的 可供个别实验室使用，价格昂贵。 该核心提供的特定翻译系统包括详细的心血管生理研究， 心血管疾病模型和病毒载体基因转移方法的发展。生理学 评估将包括：1)经胸超声心动图评估心腔大小、室壁厚度和 2)心脏收缩功能的评估，包括收缩末期的测量 压力-体积关系；3)对隔离灌流心脏的研究，这将使更精细和具体 从复杂反射激活和肾上腺素能分离的受控环境中左室收缩功能的测量 与手术准备有关的激活；4)遥测在门诊中评估血压和心电图 啮齿动物。 除了为所有项目科学家提供这些研究外，翻译系统核心还将提供 在设备齐全的集中式地点进行此类研究的高成本效益手段，避免了 需要单个参与实验室的重复努力。通过巩固生理模型 开发和评估以及病毒基因转移，节省的成本将是可观的。

The Translational Systems Core (Core C) will interface with the Project Leaders (Hammond, Insel, and Montminy), Core A (Feramisco) and Core B (Miyanohara). This shared resource is designed to provide Program scientists, in a single facility, specific techniques that are labor intensive, highly specialized, not generally available to individual laboratories and expensive. The specific translational systems provided by this Core include detailed cardiovascular physiological studies, the development of cardiovascular disease models, and virus vector gene transfer methods. Physiological assessment will include: 1) Transthoracic echocardiography to evaluate cardiac chamber size, wall thickness, and function in vivo; 2) Assessment of contractile function of the heart, including measurement of the end-systolic pressure-volume relationship; 3) The study of isolated perfused hearts, which will allow more refined and specific measurements of LV contractility in a controlled setting isolated from complex reflex activation and adrenergic activation associated with surgical preparations; 4) Telemetry to assess blood pressure and ECG in ambulatory rodents. In addition to providing these studies for all Program scientists, the Translational Systems Core will provide a highly cost efficient means to conduct such studies in a centralized and well-equipped location, circumventing the need for redundant efforts of individual participating laboratories. By consolidating physiological model development and assessment and viral gene transfer, the cost savings will be considerable.