CORE--Imaging and Physiology Core

核心D：成像和生理学核心的目标是为电子显微镜、荧光和共聚焦显微镜、图像分析和小鼠肺血管通透性测量提供技术支持、专业知识和资源，以满足所有项目的特定目标。将成像和生理支持集中在核心D内是必要的，因为个别项目强调拟议研究的生理相关性。因此，Core D将为开展和分析小鼠肺或内皮细胞单层的功能研究提供设备和技术专长。在这方面，D芯将提供专业知识和技术支持，使用生理学方法(即肺微血管过滤系数和血管壁白蛋白渗透表面积乘积)研究肺血管内皮屏障的通透性，并使用电子显微镜和定量图像分析检查特定的通透性途径。 细胞间连接和跨细胞途径的水平。所有项目都将需要由Core D提供的肺血管渗透性评估。Core D还将为含有阳离子脂质体的DNA的配方和将感兴趣的基因导入小鼠肺微血管提供专业知识。核心D人员在所述程序方面有广泛的培训和经验，将提供下述支持和资源以及 对所有项目的人员进行必要的培训。

The goal of Core D: Imaging and Physiology Core, is to provide technical support, expertise, and resources for electron microscopy, fluorescence & confocal microscopy, image analysis, and mouse lung vascular permeability measurements that will be required to address the specific aims of all projects. The centralization of the imaging and physiological support within Core D is necessitated by the emphasis of the individual projects on the physiological relevance of the proposed studies. Thus, Core D will provide equipment and technical expertise for carrying out and analyzing the functional studies in mouse lungs or endothelial cell monolayers. In this context, Core D will provide expertise and technical support for studying the permeability of lung vascular endothelial barrier using physiological methods (i.e., pulmonary microvessel filtration coefficient and vessel wall albumin permeability surface area product) as well as examination of specific permeability pathways using electron microscopy and quantitative image analysis at the level of cell-cell junctions and transcellular pathway. All Projects will require assessments of lung vascular permeability provided by Core D. Core D will also provide expertise for the formulation of DNA containing cationic liposomes and the transfection of genes of interest in mouse lung microvessels. Core D personnel, who have extensive training and experience in the described procedures, will provide the support and resources outlined below as well as the necessary training of personnel in all Projects.