Zeiss Dynamic Laser TIRF

蔡司动态激光 TIRF

• 批准号: 7217104
• 负责人: RICHARD D MINSHALL
• 金额: $ 22.45万
• 依托单位: UNIVERSITY OF ILLINOIS AT CHICAGO
• 依托单位国家: 美国
• 财政年份: 2007
• 资助国家: 美国
• 起止时间: 2007-08-01 至 2008-07-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7217104
• 关键词: Address; Adhesions; Anti-Inflammatory Agents; Anti-inflammatory; Applied Research; Area; Binding; Biological Process; Biology; Biophysics; Blood Vessels; Caveolae; Cell Adhesion; Cell membrane; Cell physiology; Cell-Cell Adhesion; Collaborations; Compatible; Complex; Cytoskeletal Proteins; Cytoskeleton; Drug Delivery Systems; Endocytosis; Endothelial Cells; Event; Exocytosis; Fluorescence Microscopy; Fostering; Functional disorder; Funding; Heart; Image; Immunology; Inflammation; Injury; Interdisciplinary Study; Investigation; Lasers; Life; Lung; Mediating; Microscope; Numbers; Pathologic Processes; Pathway interactions; Pharmaceutical Preparations; Proteins; Research; Research Personnel; Resolution; Resources; Signal Transduction; System; Technology; Therapeutic; Time; United States National Institutes of Health; Vesicle; Viral; Virus; base; cellular imaging; insight; instrumentation; interest; molecular dynamics; molecular imaging; new technology; novel strategies; particle; programs; trafficking; vascular inflammation

DESCRIPTION (provided by applicant): We are motivated to obtain a Total Internal Reflection Fluorescence Microscopy (TIRFM) system at UIC because of the realization that our understanding of complex biological processes, such as caveolae-mediated endocytosis and exocytosis, cell-cell adhesion, remodeling of the cytoskeleton, vesicle trafficking of viral particles and drug conjugates, and signal transduction requires high resolution dynamic imaging of events occurring at or near the plasma membrane. The intellectual and technological resources necessary to understand complex pathological processes such as transport, inflammation, cytoskeletal remodeling, viral entry, signaling and molecular imaging are in place, and thus the advanced TIRFM technology will be critical in helping us to proceed in our investigations as well as foster interactions among investigators with diverse but compatible interests. With availability of this new technology, we will be able to address these research questions more effectively. This application for a TIRF microscope the inter-departmental collaborations at UIC and our common interest and need for real time imaging of dynamic signaling and trafficking events. Our overall objective as a group is to understand dynamic molecular events occurring at the cell membrane. TIRF imaging will provide us with here-to-fore unavailable opportunity to delineate basic molecular interactions among key proteins involved in cell adhesion and caveolar trafficking, cell signaling, and remodeling of cytoskeletal proteins. Our strengths, as an interdisciplinary research group in basic and applied research programs in the areas of Heart and Lung Biology, Cell Physiology, and Immunology, provide the basis for the need for this instrumentation. We will be able to integrate a number of research areas and interests including pathophysiology of vascular inflammation and injury, live cell imaging, cellular trafficking, cell adhesion, and vascular biology. Relevance: The addition of the Zeiss Dynamic Laser TIRFM system will provide a unique advantage that is relevant to several key NIH funded investigators at UIC where we have nationally-recognized track-record in endothelial cell function, virus entry and vesicle trafficking, cytoskeletal protein remodeling, adhesion biophysics, signal transduction and molecular imaging. If his proposal is successful, the TIRFM technology will significantly strengthen this competitive edge. We believe that the insights gained will have the potential for pioneering novel approaches for anti-inflammatory therapeutics and drug delivery, cellular remodeling and understanding viral entry pathways.

描述（申请人提供）：我们有动力在UIC获得全内反射荧光显微镜（TIRFM）系统，因为我们认识到我们对复杂生物过程的理解，如小窝介导的内吞和胞吐，细胞-细胞粘附，细胞骨架重塑，病毒颗粒和药物缀合物的囊泡运输，并且信号转导需要对在质膜处或质膜附近发生的事件进行高分辨率动态成像。理解复杂的病理过程所需的智力和技术资源，如运输，炎症，细胞骨架重塑，病毒进入，信号传导和分子成像已经到位，因此先进的TIRFM技术将有助于我们继续进行研究，并促进具有不同但兼容兴趣的研究者之间的互动。有了这项新技术，我们将能够更有效地解决这些研究问题。TIRF显微镜的这一应用体现了UIC的跨部门合作以及我们对动态信号和贩运事件的真实的时间成像的共同兴趣和需求。作为一个小组，我们的总体目标是了解发生在细胞膜上的动态分子事件。TIRF成像将为我们提供迄今为止无法获得的机会来描绘参与细胞粘附和小窝运输、细胞信号传导和细胞骨架蛋白重塑的关键蛋白之间的基本分子相互作用。我们的优势，作为一个跨学科的研究小组在基础和应用研究计划在心脏和肺生物学，细胞生理学和免疫学领域，提供了需要这种仪器的基础。我们将能够整合一些研究领域和兴趣，包括血管炎症和损伤的病理生理学，活细胞成像，细胞运输，细胞粘附和血管生物学。相关性：蔡司动态激光TIRFM系统的加入将为UIC的几个主要NIH资助的研究人员提供独特的优势，我们在内皮细胞功能，病毒进入和囊泡运输，细胞骨架蛋白重塑，粘附生物物理学，信号转导和分子成像方面拥有国家认可的跟踪记录。如果他的建议获得成功，TIRFM技术将大大加强这种竞争优势。我们相信，所获得的见解将有可能开拓抗炎治疗和药物输送，细胞重塑和理解病毒进入途径的新方法。