CORE--Imaging and Physiology Core

核心D的目标：成像和生理学核心，是提供技术支持，专业知识和资源，电子显微镜，荧光和共聚焦显微镜，图像分析和小鼠肺血管通透性测量，将需要解决所有项目的具体目标。核心D内的成像和生理支持的集中化是必要的，因为单个项目强调了拟议研究的生理相关性。因此，核心D将提供设备和技术专长，用于在小鼠肺或内皮细胞单层中进行和分析功能研究。在此背景下，核心D将为使用生理方法研究肺血管内皮屏障的通透性提供专业知识和技术支持（即，肺微血管滤过系数和血管壁白蛋白渗透性表面积乘积）以及使用电子显微镜和定量图像分析在 细胞间连接和跨细胞通路水平。所有项目都需要由核心D提供肺血管通透性评估。核心D还将提供含有阳离子脂质体的DNA制剂和小鼠肺微血管中感兴趣基因转染的专业知识。核心D人员在所述程序方面接受过广泛培训并具有丰富经验，他们将提供下述支持和资源， 对所有项目的人员进行必要的培训。

The goal of Core D: Imaging and Physiology Core, is to provide technical support, expertise, and resources for electron microscopy, fluorescence & confocal microscopy, image analysis, and mouse lung vascular permeability measurements that will be required to address the specific aims of all projects. The centralization of the imaging and physiological support within Core D is necessitated by the emphasis of the individual projects on the physiological relevance of the proposed studies. Thus, Core D will provide equipment and technical expertise for carrying out and analyzing the functional studies in mouse lungs or endothelial cell monolayers. In this context, Core D will provide expertise and technical support for studying the permeability of lung vascular endothelial barrier using physiological methods (i.e., pulmonary microvessel filtration coefficient and vessel wall albumin permeability surface area product) as well as examination of specific permeability pathways using electron microscopy and quantitative image analysis at the level of cell-cell junctions and transcellular pathway. All Projects will require assessments of lung vascular permeability provided by Core D. Core D will also provide expertise for the formulation of DNA containing cationic liposomes and the transfection of genes of interest in mouse lung microvessels. Core D personnel, who have extensive training and experience in the described procedures, will provide the support and resources outlined below as well as the necessary training of personnel in all Projects.