Transgenic Chickens using Primordial Germ Cells

描述(由申请人提供)：Origen治疗公司的目标是开发能够产生完全人类单抗和多克隆抗体的鸡。为了实现这一目标，鸡Origen需要能够插入和修改鸡的基因组，并通过生殖系传输这些基因组修改。Origen的平台以一个基于细胞的系统为中心，该系统允许整合大段DNA并允许基因组中的精确删除。为此，Origen已经确定了一个有可能进入生殖系的细胞群体。这项应用的重点是用大的(150kb)结构来导入这个细胞群体，并敲除鸡基因组中的特定基因。然后，这些转基因细胞将通过生殖系进行交易，以获得一群转基因鸡。

DESCRIPTION (provided by applicant): Origen Therapeutic's goal is to develop chickens that produce fully human monoclonal and polyclonal antibodies. To achieve this aim the chicken Origen needs the capability to insert and modify the chicken genome and to transmit these genomic modifications through the germline. Origen's platform centers around a cell based system that allows integration of large pieces of DNA and allows precise deletions in the genome. To this end Origen has identified a cell population that has the potential to enter the germline. This application focuses on transfecting this cell population with large (150 kb) constructs and to knock-out specific genes within the chicken genome. These transfected cell popupations will then be trafficked through the germline to obtain a flock of transgenic chickens.