Novel 3-dimensional platform for high-throughput glycomics discovery

描述（由申请人提供）：糖组学是一个新兴领域，专注于研究碳水化合物与其他生物聚合物，特别是蛋白质的相互作用，以更好地了解碳水化合物在生物过程中的作用。碳水化合物微阵列技术代表了糖组学前沿的一个新发展。碳水化合物（糖缀合物，多糖，寡糖和单糖）的微阵列允许高通量，平行分析。平行分析不仅有助于研究蛋白质的碳水化合物结合活性，而且有助于阐明它们的配体。然而，由于难以可靠地固定化学和结构多样化的聚糖群体，缺乏制造高质量糖微阵列以进行高通量分析的技术。ADA建议在固体底物上开发氨基和酰肼功能化的树状大分子单层膜，用于共价、位点特异性固定未修饰的碳水化合物探针。微型微波辐射能量也将评估快速和方便地固定多糖在所提出的载玻片平台。由此产生的平台将允许简便有效地制造碳水化合物微阵列。所提出的新型载玻片平台将显著提高多糖分析的效率，满足糖组学研究和生物医学应用日益增长的需求。糖组学研究碳水化合物与其他生物聚合物，特别是蛋白质的相互作用，以更好地了解碳水化合物在生物过程中的作用。本研究旨在为生物学家开发一种新的工具，以显着提高糖组学分析的效率，满足糖组学研究和生物医学应用日益增长的需求。

DESCRIPTION (provided by applicant): Glycomics is an emerging field that focuses on studying the interactions of carbohydrates with other biopolymers, particularly proteins, to gain a better understanding of the roles of carbohydrates in biological processes. Carbohydrate microarray technologies represent a new development at the frontier of glycomics. Microarrays of carbohydrates (glycoconjugates, polysaccharides, oligosaccharides and monosaccharides) allow high-throughput, parallel analyses. Parallel analyses greatly facilitate not only surveys of proteins for carbohydrate-binding activities but also elucidation of their ligands. However, there is a lack of technology for the fabrication of high quality carbohydrate microarrays for high-throughput analysis of glycomes because of the difficulty to reliably immobilize populations of chemically and structurally diverse glycans. ADA proposes to develop aminooxy- and hydrazide-functionalized dendrimer monolayer films on solid substrates for covalent, site-specific immobilization of unmodified carbohydrate probes. Miniaturized microwave radiation energy will also be evaluated for quick and convenient immobilization of the polysaccharides on the proposed slide platform. The resulting platforms will allow facile and effective fabrication of carbohydrate microarrays. The proposed novel slide platform will significantly increase the efficiency of glycan analysis and meet the growing needs of glycomics research and biomedical applications. Glycomics study the interactions of carbohydrates with other biopolymers, particularly proteins, to gain a better understanding of the roles of carbohydrates in biological processes. This proposed research seeks to develop a new tool for biologists to significantly increase the efficiency in glycan anaysis and meet the growing needs of glycomics research and biomedical applications.